Results 21 to 30 of about 4,511 (184)

Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and Pelosinus fermentans R7 [PDF]

Genome Announcements, 2016
We and others have shown the utility of long sequence reads to improve genome assembly quality. In this study, we generated PacBio DNA sequence data to improve the assemblies of draft genomes for Clostridium thermocellum AD2, Clostridium thermocellum ...
Reddy, TBK, Varghese, Neha, Borovok, Ilya, Pillay, Manoj, Ivanova, Natalia, Hurt, Richard A, Brown, Steven D, Kyrpides, Nikos, Utturkar, Sagar M, Zhou, Jizhong, Bayer, Edward A, Markowitz, Victor, Stamatis, Dimitrios, Lamed, Raphael, Shapiro, Nicole, Woyke, Tanja, Mikhailova, Natalia, Clum, Alicia, Daum, Chris, Elias, Dwayne, Palaniappan, Krishnaveni, Huntemann, Marcel, Ngan, Chew Yee, Land, Miriam L, Klingeman, Dawn M   +24 more
core   +5 more sources

Simultaneous expression of an endogenous spermidine synthase and a butanol dehydrogenase from Thermoanaerobacter pseudethanolicus in Clostridium thermocellum results in increased resistance to acetic acid and furans, increased ethanol production and an increase in thermotolerance [PDF]

Biotechnology for Biofuels and Bioproducts, 2023
Background Sensitivity to inhibitors derived from the pretreatment of plant biomass is a barrier to the consolidated bioprocessing of these complex substrates to fuels and chemicals by microbes.
Sun-Ki Kim, Yannick J. Bomble, Janet Westpheling   +2 more
doaj   +2 more sources

Coculture with hemicellulose-fermenting microbes reverses inhibition of corn fiber solubilization by Clostridium thermocellum at elevated solids loadings [PDF]

Biotechnology for Biofuels, 2021
Background The cellulolytic thermophile Clostridium thermocellum is an important biocatalyst due to its ability to solubilize lignocellulosic feedstocks without the need for pretreatment or exogenous enzyme addition. At low concentrations of substrate, C.
Dhananjay Beri, Christopher D. Herring, Sofie Blahova, Suresh Poudel, Richard J. Giannone, Robert L. Hettich, Lee R. Lynd   +6 more
doaj   +2 more sources

Metabolic engineering of Clostridium thermocellum for n-butanol production from cellulose [PDF]

Biotechnology for Biofuels, 2019
Background Biofuel production from plant cell walls offers the potential for sustainable and economically attractive alternatives to petroleum-based products. In particular, Clostridium thermocellum is a promising host for consolidated bioprocessing (CBP)
Liang Tian, Peter M. Conway, Nicholas D. Cervenka, Jingxuan Cui, Marybeth Maloney, Daniel G. Olson, Lee R. Lynd   +6 more
doaj   +2 more sources

Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers [PDF]

Biotechnology for Biofuels and Bioproducts
Background Clostridium thermocellum is a promising candidate for production of cellulosic biofuels, however, its final product titer is too low for commercial application, and this may be due to thermodynamic limitations in glycolysis.
Bishal Dev Sharma, Shuen Hon, Eashant Thusoo, David M. Stevenson, Daniel Amador-Noguez, Adam M. Guss, Lee R. Lynd, Daniel G. Olson   +7 more
doaj   +2 more sources

Identifying promoters for gene expression in Clostridium thermocellum

Metabolic Engineering Communications, 2015
A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we test 17
Daniel G. Olson, Marybeth Maloney, Anthony A. Lanahan, Shuen Hon, Loren J. Hauser, Lee R. Lynd   +5 more
doaj   +3 more sources

Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum [PDF]

Biotechnology for Biofuels, 2012
Background Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools.
Guss Adam M, Olson Daniel G, Caiazza Nicky C, Lynd Lee R   +3 more
doaj   +3 more sources

Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum

Biotechnology for Biofuels, 2017
Background Cost-efficient saccharification is one of the main bottlenecks for industrial lignocellulose conversion. Clostridium thermocellum naturally degrades lignocellulose efficiently using the cellulosome, a multiprotein supermolecular complex, and ...
Jie Zhang, Shiyue Liu, Renmin Li, Wei Hong, Yan Xiao, Yingang Feng, Qiu Cui, Ya-Jun Liu   +7 more
doaj   +2 more sources

Atypical Glycolysis in Clostridium thermocellum [PDF]

Applied and Environmental Microbiology, 2013
Cofactor specificities of glycolytic enzymes in Clostridium thermocellum were studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP.
Deng, Y. (author), Lynd, Lee R, Argyros, D.A. (author), Argyros, D.A., Lynd, L.R., Argyros, D. Aaron, Lynd, L.R. (author), van Gulik, Walter M, Zhou, J., Olson, D.G., Zhou, Jilai, Van Gulik, W.M. (author), van Dijken, Johannes P, Olson, D.G. (author), Deng, Yu, Van Dijken, J.P., Deng, Y., Van Gulik, W.M., Olson, Daniel G, Zhou, J. (author), Van Dijken, J.P. (author)   +20 more
core   +5 more sources

Clostridium thermocellum 6

, 2015
Raw data files of Clostridium thermocellum 6<strong>Tilt Series Date:</strong> 2015-05-20</p> <strong>Data Taken By:</strong> Elitza Tocheva</p> <strong>Species / Specimen:</strong> Clostridium thermocellum&
Tocheva, Elitza
core   +6 more sources