Results 21 to 30 of about 3,327 (162)
Dcm methylation is detrimental to plasmid transformation in
Biotechnology for Biofuels, 2012 Background Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools.
Guss Adam M +3 more
doaj +3 more sources
Identifying promoters for gene expression in Clostridium thermocellum
Metabolic Engineering Communications, 2015 A key tool for metabolic engineering is the ability to express heterologous genes. One obstacle to gene expression in non-model organisms, and especially in relatively uncharacterized bacteria, is the lack of well-characterized promoters. Here we test 17
Daniel G. Olson +5 more
doaj +3 more sources
Adherence of Clostridium thermocellum to cellulose [PDF]
Journal of Bacteriology, 1983 The adherence of Clostridium thermocellum, a cellulolytic, thermophilic anaerobe, to its insoluble substrate (cellulose) was studied. The adherence phenomenon was determined to be selective for cellulose. The observed adherence was not significantly affected by various parameters, including salts, pH, temperature, detergents, or soluble sugars.
E A, Bayer, R, Kenig, R, Lamed
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Electrotransformation of Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2004 ABSTRACT Electrotransformation of several strains of Clostridium thermocellum was achieved using plasmid pIKm1 with selection based on resistance to erythromycin and lincomycin. A custom-built pulse generator was used to apply a square 10-ms pulse to an electrotransformation cuvette consisting of a ...
Michael V, Tyurin +2 more
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Atypical Glycolysis in Clostridium thermocellum [PDF]
Applied and Environmental Microbiology, 2013 ABSTRACTCofactor specificities of glycolytic enzymes inClostridium thermocellumwere studied with cellobiose-grown cells from batch cultures. Intracellular glucose was phosphorylated by glucokinase using GTP rather than ATP. Although phosphofructokinase typically uses ATP as a phosphoryl donor, we found only pyrophosphate (PPi)-linked activity ...
Zhou, J. (author) +6 more
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Form and Function of Clostridium thermocellum Biofilms [PDF]
Applied and Environmental Microbiology, 2013 ABSTRACT The importance of bacterial adherence has been acknowledged in microbial lignocellulose conversion studies; however, few reports have described the function and structure of biofilms supported by cellulosic substrates. We investigated the organization, dynamic formation, and carbon flow associated with biofilms of the ...
Alexandru, Dumitrache +4 more
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Isolation of Clostridium thermocellum Auxotrophs [PDF]
Applied and Environmental Microbiology, 1982 Spontaneous and UV irradiation-induced auxotrophic mutants of Clostridium thermocellum , an anaerobic cellulolytic thermophile, were isolated after penicillin enrichment in a chemically defined medium.
B S, Méndez, R F, Gómez
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Organization and distribution of the cellulosome in Clostridium thermocellum [PDF]
Journal of Bacteriology, 1985 The properties of the cellulosome (the cellulose-binding, multicellulase-containing protein complex) in Clostridium thermocellum were examined by comparing the cellulase systems derived from the wild type and an adherence-defective mutant. The growth conditions--specifically, growth either on cellulose (Avicel) or on cellobiose as insoluble or soluble ...
E A, Bayer, E, Setter, R, Lamed
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Characterization of Clostridium thermocellum JW20 [PDF]
Applied and Environmental Microbiology, 1988 Clostridium thermocellum JW20 (ATCC 31549), which was isolated from a Louisiana cotton bale, grew on cellulose, cellobiose, and xylooligomers and, after adaptation, on glucose, fructose, and xylose in the pH range of 7.5 to 6.1 with T opt of 60 C, T max of ...
Doris, Freier +2 more
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Elimination of formate production in Clostridium thermocellum [PDF]
Journal of Industrial Microbiology and Biotechnology, 2015 Abstract The ability of Clostridium thermocellum to rapidly degrade cellulose and ferment resulting hydrolysis products into ethanol makes it a promising platform organism for cellulosic biofuel production via consolidated bioprocessing.
Rydzak, Thomas +2 more
openaire +2 more sources