Results 41 to 50 of about 3,327 (162)

Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms

BMC Microbiology, 2012
Background Clostridium thermocellum is an anaerobic thermophilic bacterium that exhibits high levels of cellulose solublization and produces ethanol as an end product of its metabolism.
Mearls Elizabeth B, Izquierdo Javier A, Lynd Lee R   +2 more
doaj   +1 more source

Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C.
Shuen Hon, Evert K. Holwerda, Robert S. Worthen, Marybeth I. Maloney, Liang Tian, Jingxuan Cui, Paul P. Lin, Lee R. Lynd, Daniel G. Olson   +8 more
doaj   +1 more source

CP32, a novel anti‐oomycete cyclic peptide, interferes with cell‐wall deposition in Phytophthora infestans

Journal of Integrative Plant Biology, EarlyView.
The small cyclic peptide CP32 effectively inhibits Phytophthora infestans, the causal agent of potato and tomato late blight. Combined biochemical and phenotypic analyses demonstrate that CP32 disrupts cell wall biosynthesis, a key process in pathogen infection, resulting in a marked reduction of disease symptoms in plants.
Demetrio Marcianò, Stefano Rosa, Elena Marone Fassolo, Chiara Bertaso, Andrea Tagliani, Nina Gauri Capra, Federico Ballabio, Chiara Mizzotti, Giulia Rizzi, Sadia Fida Ullah, Lisa Kappel, Lucia Feni, Luca Tadini, Louise Jane Gourlay, Rosaria Russo, Vincent Bulone, Sabrina Pricl, Sandrine Ongeri, Sara Pellegrino, Carlo Camilloni, Silvia Laura Toffolatti, Vaibhav Srivastava, Paolo Pesaresi, Simona Masiero   +23 more
wiley   +1 more source

Crystal structure of endo‐β‐1,6‐galactanase from Streptomyces avermitilis

Acta Crystallographica Section D, Volume 82, Issue 8, Page 962-971, August 2026.
The crystal structure of S. avermitilis endo‐β‐1,6‐galactanase belonging to GH30 subfamily 5 reveals the first structural framework for endo‐β‐1,6‐galactanase. The β‐1,6‐galactobiose‐bound complex identifies the catalytic subsites and a distal secondary sugar‐binding site, providing insight into β‐1,6‐galactan recognition.Endo‐β‐1,6‐galactanases ...
Zui Fujimoto, Naomi Kishine, Toshihisa Kotake, Satoshi Kaneko   +3 more
wiley   +1 more source

Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems

Biotechnology for Biofuels, 2017
Background Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear.
Vanessa A. Thomas, Ninad Kothari, Samarthya Bhagia, Hannah Akinosho, Mi Li, Yunqiao Pu, Chang Geun Yoo, Sivakumar Pattathil, Michael G. Hahn, Arthur J. Raguaskas, Charles E. Wyman, Rajeev Kumar   +11 more
doaj   +1 more source

Increase in ethanol yield via elimination of lactate production in an ethanol-tolerant mutant of Clostridium thermocellum.

PLoS ONE, 2014
Large-scale production of lignocellulosic biofuel is a potential solution to sustainably meet global energy needs. One-step consolidated bioprocessing (CBP) is a potentially advantageous approach for the production of biofuels, but requires an organism ...
Ranjita Biswas, Sandeep Prabhu, Lee R Lynd, Adam M Guss   +3 more
doaj   +1 more source

Waste to Hydrogen: Transforming Food Waste Into Biohythane (Bio‐H2 + Bio‐CH4) in a Two‐Stage Reactor With the Aid of a Metal‐Ion Catalyst

Energy Science &Engineering, Volume 14, Issue 7, Page 3388-3421, July 2026.
This study demonstrates a two‐stage catalytic bioreactor system that converts real food waste into high‐purity biohydrogen and biohythane. In Stage‐1, an enriched Clostridium thermocellum culture combined with Ni2+─Fe2+ bimetallic catalysis enhances hydrolysis efficiency and hydrogenase activity, resulting in a 77% increase in H2 yield and 75.8% purity
K. V. Sreedharan, Debabrata Barik, Ayyar Dinesh, D. Shanmugapriya, M. Santhamoorthy, N. Ashok   +5 more
wiley   +1 more source

Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol

Biotechnology for Biofuels, 2017
Background Clostridium thermocellum is a promising microorganism for conversion of cellulosic biomass to biofuel, without added enzymes; however, the low ethanol titer produced by strains developed thus far is an obstacle to industrial application ...
Liang Tian, Skyler J. Perot, David Stevenson, Tyler Jacobson, Anthony A. Lanahan, Daniel Amador-Noguez, Daniel G. Olson, Lee R. Lynd   +7 more
doaj   +1 more source

Integrative SAXS and AFM analysis of engineered carbohydrate‐active enzyme assemblies with tunable spatial organization

Protein Science, Volume 35, Issue 7, July 2026.
Abstract Cellulosomes are multi‐enzyme assemblies whose catalytic efficiency depends on the spatial organization of their components. However, their pronounced conformational flexibility has precluded quantitative characterization of inter‐enzyme distances and overall topology.
Iker Pardo Larrabeiti, Manuel Eibinger, Jeremy Esque, Stéphanie Pradeau, Sébastien Fort, Sarah Moraïs, Itzhak Mizrahi, Edward A. Bayer, Bernd Nidetzky, Cédric Y. Montanier, Pierre Roblin, Claire Dumon   +11 more
wiley   +1 more source

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose‐Degrading Anaerobic Bacteria

Angewandte Chemie, Volume 137, Issue 24, June 10, 2025.
The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Keishi Ishida, Jana Krabbe, Philippe R. Meisinger, Gulimila Shabuer, Sebastian Schieferdecker, Michael Cyrulies, Cedric Tank, Emma Barnes, Christian Paetz, Christian Hertweck   +9 more
wiley   +2 more sources