Results 41 to 50 of about 4,511 (184)

Tracking the cellulolytic activity of Clostridium thermocellum biofilms [PDF]

Biotechnology for Biofuels, 2013
Background Microbial cellulose conversion by Clostridium thermocellum 27405 occurs predominantly through the activity of substrate-adherent bacteria organized in thin, primarily single cell-layered biofilms. The importance of cellulosic surface exposure to microbial hydrolysis has received little attention despite its implied impact on conversion ...
Dumitrache, Alexandru, Wolfaardt, Gideon M., Allen, David Grand, Liss, Steven N., Lynd, Lee R.   +4 more
openaire   +4 more sources

Glycolysis without pyruvate kinase in Clostridium thermocellum [PDF]

Metabolic Engineering, 2017
The metabolism of Clostridium thermocellum is notable in that it assimilates sugar via the EMP pathway but does not possess a pyruvate kinase enzyme. In the wild type organism, there are three proposed pathways for conversion of phosphoenolpyruvate (PEP) to pyruvate, which differ in their cofactor usage. One path uses pyruvate phosphate dikinase (PPDK),
Olson DG, Hörl M, Fuhrer T, Cui J, Zhou J, Maloney MI, Amador-Noguez D, Tian L, Sauer U, Lynd LR   +9 more
openaire   +3 more sources

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature

Metabolic Engineering Communications, 2016
Clostridium thermocellum is a leading candidate for the consolidated bioprocessing of lignocellulosic biomass for the production of fuels and chemicals.
Joseph Groom, Daehwan Chung, Daniel G. Olson, Lee R. Lynd, Adam M. Guss, Janet Westpheling   +5 more
doaj   +1 more source

A Blue Native-PAGE analysis of membrane protein complexes in Clostridium thermocellum

BMC Microbiology, 2011
Background Clostridium thermocellum is a Gram-positive thermophilic anaerobic bacterium with the unusual capacity to convert cellulosic biomass into ethanol and hydrogen. Identification and characterization of protein complexes in C.
Fan Keqiang, Xu Xinping, Yu Tingting, Luo Yuanming, Peng Yanfeng, Zhao Youbao, Yang Keqian   +6 more
doaj   +1 more source

Expressing the Thermoanaerobacterium saccharolyticum pforA in engineered Clostridium thermocellum improves ethanol production

Biotechnology for Biofuels, 2018
Background Clostridium thermocellum has been the subject of multiple metabolic engineering strategies to improve its ability to ferment cellulose to ethanol, with varying degrees of success. For ethanol production in C.
Shuen Hon, Evert K. Holwerda, Robert S. Worthen, Marybeth I. Maloney, Liang Tian, Jingxuan Cui, Paul P. Lin, Lee R. Lynd, Daniel G. Olson   +8 more
doaj   +1 more source

Discovery and Biosynthesis of Celluxanthenes, Antibacterial Arylpolyene Alkaloids From Diverse Cellulose‐Degrading Anaerobic Bacteria

Angewandte Chemie, Volume 137, Issue 24, June 10, 2025.
The structure and biosynthetic origin of the enigmatic “yellow affinity substance” (YAS) from the anaerobe thermophile Clostridium thermocellum (now: Acetivibrio thermocellus) were elucidated. YAS is a complex of unusual antibacterial arylpolyene alkaloids (celluxanthenes) that are produced by various cellulolytic anaerobes.
Keishi Ishida, Jana Krabbe, Philippe R. Meisinger, Gulimila Shabuer, Sebastian Schieferdecker, Michael Cyrulies, Cedric Tank, Emma Barnes, Christian Paetz, Christian Hertweck   +9 more
wiley   +2 more sources

Coproduction of hydrogen and volatile fatty acid via thermophilic fermentation of sweet sorghum stalk from co-culture of Clostridium thermocellum and Clostridium thermosaccharolyticum

, 2017
This study was focused on investigating the potential of hydrogen and volatile fatty acid (VFA) coproduction. Sweet sorghum stalks (SS) were used as substrate along with Clostridium thermocellum and Clostridium thermosaccharolyticum as production ...
Guo, Chen, Sui, Kun-Yan, Liu, Chun-Zhao, Zhang, Chen, Islam, Md. Saiful   +4 more
core   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, Volume 16, Issue 4, Page 653-669, April 2026.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Logic‐Gated HSV‐TK/GCV Suicide Gene Circuit for Triple‐Negative Breast Cancer

Advanced Science, Volume 13, Issue 20, 9 April 2026.
The BRAS comprises two modular genetic components driven by distinct tumor‐specific promoters and a failsafe layer with the NOT gate. This multi‐input logic gate circuit enables precise, specific expression of HSV‐TK in breast cancer cells with hardly expression in normal cell and effectively inhibits tumor growth in a triple‐negative breast cancer ...
Shasha Tang, Yuan Fang, Lingli Jin, Dongyang Liu, Yicheng Liu, Ruijia Zheng, Liyun Yong, Xin Wu, Longliang Qiao, Meiyan Wang, Fengfeng Cai   +10 more
wiley   +1 more source

Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems

Biotechnology for Biofuels, 2017
Background Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear.
Vanessa A. Thomas, Ninad Kothari, Samarthya Bhagia, Hannah Akinosho, Mi Li, Yunqiao Pu, Chang Geun Yoo, Sivakumar Pattathil, Michael G. Hahn, Arthur J. Raguaskas, Charles E. Wyman, Rajeev Kumar   +11 more
doaj   +1 more source