Comparison of the Quality of Protein Crystals Grown by CLPC Seeds Method
Crystals, 2019 We present a systematic quality comparison of protein crystals obtained with and without cross-linked protein crystal (CLPC) seeds. Four proteins were used to conduct the experiments, and the results showed that crystals obtained in the presence of CLPC ...
Zi-Qing Wu, Xudong Deng, Ya-Li Liu
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Allosteric control of the bacterial ClpC/ClpP protease and its hijacking by antibacterial peptides [PDF]
The EMBO JournalThe hexameric AAA+ protein ClpC, combined with peptidase ClpP, forms a critical ATP-dependent protease in bacteria, essential for virulence. ClpC is usually repressed in an inactive resting state, where two ClpC spirals interact via coiled-coil M-domains.
Timo Jenne +13 more
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Journal of Global Antimicrobial Resistance, 2023 : Objectives: Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation.
Osvaldo Inostroza +2 more
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clpC-Mediated Translational Control Orchestrates Stress Tolerance and Biofilm Formation in Milk-Originated Staphylococcus aureus RMSA24 [PDF]
FoodsStaphylococcus aureus is an important pathogen that can cause widespread infections as well as severe outbreaks of food poisoning. Recent studies have drawn attention to foodborne pathogens such as S.
Maofeng Zhang, Jie Hu, Ting Xue
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Septic Shock Caused by Coinfection of Shewanella algae Bloodstream Infection and Epstein‐Barr Virus: Clinical Characteristics and Genomic Analysis [PDF]
MicrobiologyOpenShewanella algae, a marine‐origin opportunistic pathogen, has shown a significant increase in non‐coastal infections, yet its environmental adaptability and synergistic pathogenic mechanisms with Epstein‐Barr virus (EBV) coinfection remain unclear.
Jianmei Chen +6 more
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Structure of the central Staphylococcus aureus AAA+ protease MecA/ClpC/ClpP [PDF]
Communications BiologyBacterial AAA+ proteases are composed of a AAA+ partner (e.g., ClpC) and an associated peptidase (e.g., ClpP). They represent ATP-fuelled and self-compartmentalized proteolytic machines that are crucial for stress resistance and virulence.
Stavros Azinas +6 more
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Components of the Clp complex are required for sporangium formation and dehiscence in Actinoplanes missouriensis [PDF]
Journal of BacteriologyThe actinomycete Actinoplanes missouriensis forms terminal sporangia that contain a few hundred spores. When immersed in water, the sporangium opens to release spores, which start swimming using flagella, via a process referred to as sporangium ...
Ryota Suzuki +4 more
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Thioredoxin and its partner protein are essential for zoospore flagellar formation in Actinoplanes missouriensis [PDF]
Communications BiologyThe filamentous bacterium Actinoplanes missouriensis produces flagellated spores, which become zoospores after release, in sporangia. However, the regulatory mechanism of spore flagellation remains unclear.
Tomohiro Kimura +4 more
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Overexpressing the ClpC AAA+ unfoldase accelerates developmental cycle progression in Chlamydia trachomatis [PDF]
mBioChlamydia is an obligate intracellular bacterium that undergoes a complex biphasic developmental cycle, alternating between the smaller, infectious, non-dividing elementary body (EB) and the larger, non-infectious but dividing reticulate body. Due to the
Aaron A. Jensen +4 more
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ApoE Mimetic Peptide COG1410 Kills Mycobacterium smegmatis via Directly Interfering ClpC’s ATPase Activity [PDF]
AntibioticsAntimicrobial peptides (AMPs) hold promise as alternatives to combat bacterial infections, addressing the urgent global threat of antibiotic resistance.
Chun Wang +10 more
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