Identification of substrates of the human mitochondrial ClpXP protease and its implications for Perrault syndrome [PDF]
Nature CommunicationsThe ClpXP ATP-dependent unfoldase-protease complex plays important roles in maintaining protein homeostasis in the mitochondria. Pathogenic variants of CLPP have been identified in patients with Perrault syndrome type 3 (PRLTS3), a rare recessive ...
Mazen E. Aljghami +14 more
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Cryo-EM structures of human ClpXP reveal mechanisms of assembly and proteolytic activation [PDF]
Nature CommunicationsThe human ClpXP complex (hClpXP) orchestrates mitochondrial protein quality control through targeted degradation of misfolded and unnecessary proteins.
Wenqian Chen +2 more
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The ClpXP protease and the ClpX unfoldase control virulence, cell division, and autolysis in Streptococcus pneumoniae [PDF]
Microbiology SpectrumIn all living cells, molecular chaperones and ATP-dependent proteases are essential for protein homeostasis. The ClpXP protease is a chaperone-protease complex conserved between bacteria and mitochondria.
Viktor H. Mebus +7 more
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Frontiers in Molecular Biosciences, 2017 The ClpXP protease is a highly conserved AAA+ degradation machine that is present throughout bacteria and in eukaryotic organelles. ClpXP is essential in some bacteria, such as Caulobacter crescentus, but dispensible in others, such as Escherichia coli ...
Peter Chien
exaly +3 more sources
A new regulator of the Staphylococcus aureus peptidoglycan hydrolase Sle1. [PDF]
PLoS GeneticsRegulation of peptidoglycan hydrolases is crucial for bacterial cell integrity, growth and division. In the bacterial pathogen Staphylococcus aureus, the amidase Sle1 is a key autolysin required for septum splitting and daughter cell separation.
Helena Veiga +3 more
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Temperature-dependent regulation of bacterial cell division hydrolases by the coordinated action of a regulatory RNA and the ClpXP protease [PDF]
The Cell SurfaceA defining feature of bacteria is the peptidoglycan cell wall which provides structural integrity and prevents osmotic lysis. While peptidoglycan hydrolases are required for daughter cell separation, dysregulated cell wall degradation may result in cell ...
Viktor H. Mebus +14 more
doaj +2 more sources
Utilizing nanoinducers for precision degradation of bacterial protein to mitigate antibiotic resistance [PDF]
Nature CommunicationsAntibiotic resistance stands as a formidable global challenge to public health. Herein, we present a bacterial nanoinducer (bacNID) designed for targeted protein degradation in treating bacterial infections. Specifically, bacNID is engineered by grafting
Linqi Han +6 more
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Targeted protein degradation in Escherichia coli using CLIPPERs [PDF]
EMBO ReportsNew, universal tools for targeted protein degradation in bacteria can help to accelerate protein function studies and antimicrobial research. We describe a new method for degrading bacterial proteins using plasmid-encoded degrader peptides which deliver ...
Matylda Anna Izert-Nowakowska +9 more
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Role of ClpX and MsrA/B in the Oxidative and Cell Wall Stress Response in Bacillus anthracis Sterne [PDF]
MicrobiologyOpenClpX functions as a component of the ClpXP protease, a conserved intracellular protease that regulates protein turnover, stress responses, and virulence in multiple bacterial species.
Aeron B. Pennington +5 more
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Cellular functions of the ClpP protease impacting bacterial virulence
Frontiers in Molecular Biosciences, 2022 Proteostasis mechanisms significantly contribute to the sculpting of the proteomes of all living organisms. ClpXP is a central AAA+ chaperone-protease complex present in both prokaryotes and eukaryotes that facilitates the unfolding and subsequent ...
Mazen E. Aljghami +5 more
doaj +1 more source