Results 281 to 290 of about 161,459 (317)

Are endogenous clustered dna damages induced in human cells?

Free Radical Biology and Medicine, 2004
Although clustered DNA damages are induced in cells by ionizing radiation and can be induced artifactually during DNA isolation, it was not known if they are formed in unirradiated cells by normal oxidative metabolism. Using high-sensitivity methods of quantitative gel electrophoresis, electronic imaging, and number average length analysis, we found ...
Paula V, Bennett, Nela S, Cintron, Laurent, Gros, Jacques, Laval, Betsy M, Sutherland   +4 more
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Oxidatively generated complex DNA damage: Tandem and clustered lesions

Cancer Letters, 2012
There is an increasing interest for oxidatively generated complex lesions that are potentially more detrimental than single oxidized nucleobases. In this survey, the recently available information on the formation and processing of several classes of complex DNA damage formed upon one radical hit including mostly hydroxyl radical and one-electron ...
Cadet, J., Ravanat, J.-L., Taverna Porro, M., Menoni, H., Angelov, D.   +4 more
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Clustered DNA damage on subcellular level: effect of scavengers

Radiation and Environmental Biophysics, 2014
Clustered DNA damages are induced by ionizing radiation, particularly of high linear energy transfer (LET). Compared to isolated DNA damage sites, their biological effects can be more severe. We investigated a clustered DNA damage induced by high LET radiation (C 290 MeV u(-1) and Fe 500 MeV u(-1)) in pBR322 plasmid DNA.
Kateřina, Pachnerová Brabcová, Lembit, Sihver, Nakahiro, Yasuda, Youichirou, Matuo, Václav, Stěpán, Marie, Davídková   +5 more
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Clustered DNA Damages Induced by X Rays in Human Cells

Radiation Research, 2002
Although DNA DSBs are known to be important in producing the damaging effects of ionizing radiation in cells, bistranded clustered DNA damages-two or more oxidized bases, abasic sites or strand breaks on opposing DNA strands within a few helical turns-are postulated to be difficult to repair and thus to be critical radiation-induced lesions. Gamma rays
Betsy M, Sutherland, Paula V, Bennett, John C, Sutherland, Jacques, Laval   +3 more
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A nanodosimetric model of radiation-induced clustered DNA damage yields

Physics in Medicine and Biology, 2010
We present a nanodosimetric model for predicting the yield of double strand breaks (DSBs) and non-DSB clustered damages induced in irradiated DNA. The model uses experimental ionization cluster size distributions measured in a gas model by an ion counting nanodosimeter or, alternatively, distributions simulated by a Monte Carlo track structure code ...
Garty, G., Schulte, R., Shchemelinin, S., Leloup, Corinne, Assaf, G., Breskin, A., Chechik, R., Bashkirov, V., Miligan, J., Grosswendt, B.   +9 more
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Clustered DNA Damage and its Complexity: Tracking the History

Radiation Research
The concept of radiation-induced clustered damage in DNA has grown over the past several decades to become a topic of considerable interest across the scientific disciplines involved in studies of the biological effects of ionizing radiation. This paper, prepared for the 70th anniversary issue of Radiation Research, traces historical development of the
Dudley T, Goodhead, Michael, Weinfeld
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Effect of Hydroxyl Radical Scavenging Capacity on Clustering of DNA Damage

Radiation Research, 1997
We have shown previously that the thiol N-(2'-mercaptoethyl)-1,3-diaminopropane (WR-1065) can attenuate the formation of strand breaks associated with ionizing radiation. The mechanism of this protection is predominantly the reduction of DNA radical species which otherwise would attenuate the chemical repair of DNA radical species which are strand ...
J R, Milligan, J A, Aguilera, C C, Wu, R A, Paglinawan, T T, Nguyen, D, Wu, J F, Ward   +6 more
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Endogenous DNA damage clusters in human hematopoietic stem and progenitor cells

Free Radical Biology and Medicine, 2008
Clustered DNA damages-multiple oxidized bases, abasic sites, or strand breaks within a few helical turns-are potentially mutagenic and lethal alterations induced by ionizing radiation. Endogenous clusters are found at low frequencies in unirradiated normal human cells and tissues. Radiation-sensitive hematopoietic cells with low glycosylase levels (TK6
Paula, Bennett, Alexander A, Ishchenko, Jacques, Laval, Brigitte, Paap, Betsy M, Sutherland   +4 more
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Condensation of DNA—A putative obstruction for repair process in abasic clustered DNA damage

DNA Repair, 2013
Clustered DNA damages are defined as two or more closely located DNA damage lesions that may be present within a few helical turns of the DNA double strand. These damages are potential signatures of ionizing radiation and are often found to be repair resistant. Types of damaged lesions frequently found inside clustered DNA damage sites include oxidized
Vandana, Singh, Prolay, Das
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Simulation of Proton-Induced DNA Damage Patterns Using an Improved Clustering Algorithm

Radiation Research, 2020
Simulations of deoxyribonucleic acid (DNA) molecular damage use the traversal algorithm that has the disadvantages of being time-consuming, slowly converging, and requiring high-performance computer clusters. This work presents an improved version of the algorithm, "density-based spatial clustering of applications with noise" (DBSCAN), using a KD-tree ...
Jing, Tang, Qinfeng, Xiao, Zhiguo, Gui, Baosheng, Li, Pengcheng, Zhang   +4 more
openaire   +2 more sources