Results 1 to 10 of about 204,351 (287)

Extended stop codon context predicts nonsense codon readthrough efficiency in human cells

Nature Communications
Protein synthesis terminates when a stop codon enters the ribosome’s A-site. Although termination is efficient, stop codon readthrough can occur when a near-cognate tRNA outcompetes release factors during decoding.
Kotchaphorn Mangkalaphiban, Lianwu Fu, Ming Du, Kari Thrasher, Kim M. Keeling, David M. Bedwell, Allan Jacobson   +6 more
doaj   +4 more sources

Translational readthrough of nonsense mutant TP53 by mRNA incorporation of 5-Fluorouridine

Cell Death and Disease, 2022
TP53 nonsense mutations in cancer produce truncated inactive p53 protein. We show that 5-FU metabolite 5-Fluorouridine (FUr) induces full-length p53 in human tumor cells carrying R213X nonsense mutant TP53.
Mireia Palomar-Siles, Angelos Heldin, Meiqiongzi Zhang, Charlotte Strandgren, Viktor Yurevych, Jip T. van Dinter, Sem A. G. Engels, Damon A. Hofman, Susanne Öhlin, Birthe Meineke, Vladimir J. N. Bykov, Sebastiaan van Heesch, Klas G. Wiman   +12 more
doaj   +2 more sources

2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations

Nature Communications, 2020
Nonsense mutations can be corrected by several molecules that activate readthrough of premature termination codon. Here, the authors report that 2,6-diaminopurine efficiently corrects UGA nonsense mutations with no significant toxicity.
Carole Trzaska, Séverine Amand, Christine Bailly, Catherine Leroy, Virginie Marchand, Evelyne Duvernois-Berthet, Jean-Michel Saliou, Hana Benhabiles, Elisabeth Werkmeister, Thierry Chassat, Romain Guilbert, David Hannebique, Anthony Mouray, Marie-Christine Copin, Pierre-Arthur Moreau, Eric Adriaenssens, Andreas Kulozik, Eric Westhof, David Tulasne, Yuri Motorin, Sylvie Rebuffat, Fabrice Lejeune   +21 more
doaj   +2 more sources

Interaction of nonsense suppressor tRNAs and codon nonsense mutations or termination codons

Advances in Biological Chemistry, 2012
Codon nonsense mutations include amber, ochre, or opal mutations according to termination codon consisting of three types (TAG, TAA and TGA). Codon nonsense mutations are also divided into natural and artificial mutations. We discussed the interaction of codon nonsense mutations and suppressor tRNAs in vitro and in vivo.
Lu Zixian
openaire   +3 more sources

Codon usage in vertebrates is associated with a low risk of acquiring nonsense mutations

Journal of Translational Medicine, 2011
Background Codon usage in genomes is biased towards specific subsets of codons. Codon usage bias affects translational speed and accuracy, and it is associated with the tRNA levels and the GC content of the genome.
Flegel Willy A, Schmid Pirmin
doaj   +2 more sources

Translation velocity determines the efficacy of engineered suppressor tRNAs on pathogenic nonsense mutations

Nature Communications
Nonsense mutations – the underlying cause of approximately 11% of all genetic diseases – prematurely terminate protein synthesis by mutating a sense codon to a premature stop or termination codon (PTC).
Nikhil Bharti, Leonardo Santos, Marcos Davyt, Stine Behrmann, Marie Eichholtz, Alejandro Jimenez-Sanchez, Jeong S. Hong, Andras Rab, Eric J. Sorscher, Suki Albers, Zoya Ignatova   +10 more
doaj   +2 more sources

Nonsynonymous, synonymous and nonsense mutations in human cancer-related genes undergo stronger purifying selections than expectation

BMC Cancer, 2019
Background Nonsynonymous mutations change the protein sequences and are frequently subjected to natural selection. The same goes for nonsense mutations that introduce pre-mature stop codons into CDSs (coding sequences). Synonymous mutations, however, are
Duan Chu, Lai Wei
doaj   +2 more sources

AAV-delivered engineered suppressor tRNA rescues visual function in mice with an inherited retinal disease [PDF]

Nature Communications
Nonsense mutations change a sense codon into a premature termination codon (PTC) in mRNA and account for approximately 18.5% of human inherited retinal diseases (IRDs)-related mutation.
Chengda Ren, Li Song, Ming Hu, Xiu Jin, Licong Liang, Fanfei Liu, Jing Su, Jiamei Fu, Yiliu Yang, Man Liu, Qiuxia Xu, Xiaoyi Wu, Min Luo, Qin Ye, Qiqi Li, Yifang An, Manjun Li, Kaiqin She, Yuquan Wei, Fang Lu, Yang Yang   +20 more
doaj   +2 more sources

Intranuclear degradation of nonsense codon‐containing mRNA [PDF]

EMBO reports, 2002
Most vertebrate mRNAs with premature termination codons (PTCs) are specifically recognized and degraded by a process referred to as nonsense‐mediated mRNA decay (NMD) while still associated with the nucleus. However, it is still a matter of debate whether PTCs can be identified by intranuclear scanning or only by ribosomes on the cytoplasmic side of ...
Bühler, Marc, Wilkinson, Miles F., Mühlemann, Oliver   +2 more
openaire   +3 more sources

CGG: an unassigned or nonsense codon in Mycoplasma capricolum. [PDF]

Proceedings of the National Academy of Sciences, 1991
CGG is an arginine codon in the universal genetic code. We previously reported that in Mycoplasma capricolum, a relative of Gram-positive eubacteria, codon CGG did not appear in coding frames, including termination sites, and tRNA(ArgCCG) pairing with codon CGG, was not detected. These facts suggest that CGG is a nonsense (unassigned and untranslatable)
T, Oba, Y, Andachi, A, Muto, S, Osawa
openaire   +3 more sources