Codon usage in vertebrates is associated with a low risk of acquiring nonsense mutations [PDF]
Background Codon usage in genomes is biased towards specific subsets of codons. Codon usage bias affects translational speed and accuracy, and it is associated with the tRNA levels and the GC content of the genome.
Flegel Willy A, Schmid Pirmin
doaj +5 more sources
Translational readthrough of nonsense mutant TP53 by mRNA incorporation of 5-Fluorouridine
TP53 nonsense mutations in cancer produce truncated inactive p53 protein. We show that 5-FU metabolite 5-Fluorouridine (FUr) induces full-length p53 in human tumor cells carrying R213X nonsense mutant TP53.
Mireia Palomar-Siles+12 more
doaj +2 more sources
Interaction of nonsense suppressor tRNAs and codon nonsense mutations or termination codons
Codon nonsense mutations include amber, ochre, or opal mutations according to termination codon consisting of three types (TAG, TAA and TGA). Codon nonsense mutations are also divided into natural and artificial mutations. We discussed the interaction of
Lu Zixian
semanticscholar +4 more sources
2,6-Diaminopurine as a highly potent corrector of UGA nonsense mutations
Nonsense mutations can be corrected by several molecules that activate readthrough of premature termination codon. Here, the authors report that 2,6-diaminopurine efficiently corrects UGA nonsense mutations with no significant toxicity.
Carole Trzaska+21 more
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Background Nonsynonymous mutations change the protein sequences and are frequently subjected to natural selection. The same goes for nonsense mutations that introduce pre-mature stop codons into CDSs (coding sequences). Synonymous mutations, however, are
Duan Chu, Lai Wei
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A G542X cystic fibrosis mouse model for examining nonsense mutation directed therapies. [PDF]
Nonsense mutations are present in 10% of patients with CF, produce a premature termination codon in CFTR mRNA causing early termination of translation, and lead to lack of CFTR function.
Daniel R McHugh+9 more
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CGG: an unassigned or nonsense codon in Mycoplasma capricolum. [PDF]
CGG is an arginine codon in the universal genetic code. We previously reported that in Mycoplasma capricolum, a relative of Gram-positive eubacteria, codon CGG did not appear in coding frames, including termination sites, and tRNA(ArgCCG) pairing with ...
Takanori Oba+3 more
semanticscholar +4 more sources
Extended stop codon context predicts nonsense codon readthrough efficiency in human cells
Protein synthesis terminates when a stop codon enters the ribosome’s A-site. Although termination is efficient, stop codon readthrough can occur when a near-cognate tRNA outcompetes release factors during decoding.
Kotchaphorn Mangkalaphiban+6 more
doaj +3 more sources
Infrequent translation of a nonsense codon is sufficient to decrease mRNA level. [PDF]
In many organisms nonsense mutations decrease the level of mRNA. In the case of mammalian cells, it is still controversial whether translation is required for this nonsense-mediated RNA decrease (NMD).
Alla Buzina, Marc J. Shulman
semanticscholar +3 more sources
Nonsense Mutations in Close Proximity to the Initiation Codon Fail to Trigger Full Nonsense-mediated mRNA Decay* [PDF]
Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs containing premature translation termination codons. In mammalian cells, a termination codon is ordinarily recognized as "premature" if it is located greater than 50-54 nucleotides 5' to the final exon-exon junction.
Â. Inácio+9 more
semanticscholar +5 more sources