Results 111 to 120 of about 204,351 (287)
AUG sequences are required to sustain nonsense-codon-mediated suppression of splicing [PDF]
Nucleic Acids Research, 2006 More than 90% of human genes are rich in intronic latent 5' splice sites whose utilization in pre-mRNA splicing would introduce in-frame stop codons into the resultant mRNAs. We have therefore hypothesized that suppression of splicing (SOS) at latent 5' splice sites regulates alternative 5' splice site selection in a way that prevents the production of
Kamhi, Eyal +5 more
openaire +2 more sources
Tyrosine decaging leads to substantial membrane trafficking during modulation of an inward rectifier potassium channel [PDF]
, 2001 Tyrosine side chains participate in several distinct signaling pathways, including phosphorylation and membrane trafficking. A nonsense suppression procedure was used to incorporate a caged tyrosine residue in place of the natural tyrosine at position ...
Brandt, Gabriel S. +7 more
core +3 more sources
Genetic Diversity, Adaptation, Wild Introgression, and Coat Color Mutation of Golden Yak
Animal Research and One Health, EarlyView.Genetic diversity, adaptation, wild introgression, and coat color mutation of golden yak from two populations on the Qinghai‐Xizang Plateau. ABSTRACT The golden yak lives on the Qinghai‐Xizang Plateau with a golden coat and adapts to high altitudes and strong ultraviolet environment. The golden coat is a prominent phenotype in many domesticated species,
Huixuan Yan +15 more
wiley +1 more source
A Novel G542X CFTR Rat Model of Cystic Fibrosis Is Sensitive to Nonsense Mediated Decay
Frontiers in Physiology, 2020 Nonsense mutations that lead to the insertion of a premature termination codon (PTC) in the cystic fibrosis transmembrane conductance regulator (CFTR) transcript affect 11% of patients with cystic fibrosis (CF) worldwide and are associated with severe ...
Jyoti Sharma +8 more
doaj +1 more source
Biotechnology and Bioengineering, EarlyView.A novel puromycin linker for cDNA display was synthesized via SPAAC reaction. Its functionality was confirmed by recovering intact DNA after selection against EpCAM. The system also enabled the verification of bicyclic peptides during display, demonstrating its utility for constructing and screening structurally complex peptide libraries.
Simon Schneider +3 more
wiley +1 more source
Rapidly evolving protointrons in Saccharomyces genomes revealed by a hungry spliceosome. [PDF]
, 2019 Introns are a prevalent feature of eukaryotic genomes, yet their origins and contributions to genome function and evolution remain mysterious. In budding yeast, repression of the highly transcribed intron-containing ribosomal protein genes (RPGs ...
Ares, Manuel +8 more
core +1 more source
Nucleic Acids Research, 1994 Nonsense suppressor tRNAs have been suggested as potential agents for human somatic gene therapy. Recent work from this laboratory has described significant effects of 3' codon context on the efficiency of human nonsense suppressors. A rapid increase in the number of reports of human diseases caused by nonsense codons, prompted us to determine how the ...
J, Atkinson, R, Martin
openaire +3 more sources
The power of many: when genetics met yeasts and high‐throughput
Biological Reviews, EarlyView.ABSTRACT In recent years, complex technological capabilities have evolved, driven by the need to solve complex and integrative biological questions through global analyses. New equipment allows the scaling up and automation of processes which previously were carried out on a very limited scale.
Víctor A. Tallada, Víctor Carranco
wiley +1 more source
A minor alternative transcript of the fumarylacetoacetate hydrolase gene produces a protein despite being likely subjected to nonsense-mediated mRNA decay [PDF]
, 2005 BACKGROUND: Coupling of alternative splicing with nonsense-mediated mRNA decay (NMD) may regulate gene expression. We report here the identification of a nonsense alternative transcript of the fumarylacetoacetate hydrolase (fah) gene, which produces a ...
Bergeron, Anne +4 more
core +3 more sources
The influence of the reading context upon the suppression of nonsense codons
Molecular and General Genetics MGG, 1969 We have examined the response of phage T4 nonsense mutations located at various sites within the same cistron to different suppression agents. A wide range of suppression efficiency is found for both ochre (UAA) and amber (UAG) mutations under conditions where suppression provides a measurement of the amount of chain propagation past the mutated site ...
M M, Fluck, W, Salser, R H, Epstein
openaire +4 more sources