Results 81 to 90 of about 69,281 (285)
Visualization of codon-dependent conformational rearrangements during translation termination [PDF]
Nature Structural & Molecular Biology, 2010 Although the recognition of stop codons by class 1 release factors (RFs) on the ribosome takes place with extremely high fidelity, the molecular mechanisms behind this remarkable process are poorly understood. Here we performed structural probing experiments with Fe(II)-derivatized RFs to compare the conformations of cognate and near-cognate ribosome ...
Shan L, He, Rachel, Green
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Advanced Science, EarlyView.ABSTRACT Orthogonal translation systems (OTSs) enable site‐specific incorporation of non‐canonical amino acids (ncAAs) and are central to genetic code expansion. Current engineering strategies typically rely on hyperstable aminoacyl tRNA synthetase (aaRS) scaffolds to tolerate destabilizing mutations required for substrate diversification.
Nikolaj G. Koch +4 more
wiley +1 more source
Thermodynamic and kinetic insights into stop codon recognition by release factor 1. [PDF]
, 2014 Stop codon recognition is a crucial event during translation termination and is performed by class I release factors (RF1 and RF2 in bacterial cells). Recent crystal structures showed that stop codon recognition is achieved mainly through a network of ...
Joseph, Simpson +2 more
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A 22-Week-Old Fetus with Nager Syndrome and Congenital Diaphragmatic Hernia due to a Novel SF3B4 Mutation. [PDF]
, 2014 Nager syndrome, or acrofacial dysostosis type 1 (AFD1), is a rare multiple malformation syndrome characterized by hypoplasia of first and second branchial arches derivatives and appendicular anomalies with variable involvement of the radial/axial ray. In
BOTTILLO, IRENE +7 more
core +1 more source
Termination Codons and Stop Codon Context in Bacteria and Mammalian Mitochondria
Biotechnology & Biotechnological Equipment, 2013 ABSTRACTThe aim of this study was to analyze the frequency of occurrence of individual stop codons and combinations of stop codons with adjacent upstream and downstream triplets in 264 bacterial and 1308 mammalian mitochondrial genomes. For the purpose of this analysis a novel program (Gene Triplet Analysis) was applied.
Kiril T. Kirilov +2 more
openaire +1 more source
Advanced Science, EarlyView.TRMT6‐mediated m1A modification in CDK9 mRNA enhances its mRNA stability and translation efficiency, thereby increasing the protein levels of CDK9. Upregulated CDK9 promotes the progression of HCC by elevating the levels of oncogenic factors including p‐STAT3, MCL1, and BCL‐2. On the other hand, CDK9 phosphorylates TARDBP at Ser254 to activate HBV core
Rui Zhang +12 more
wiley +1 more source
Engineering Synthetic Antibody by Expanded Genetic Code [PDF]
, 2017 Antibodies are extensively used in research for diagnostic and therapeutic purposes because of their unrivaled specificity and biomarker binding strengths.1 Currently, monoclonal antibodies are most commonly used because of their production consistency ...
Batiuk, Elizabeth +2 more
core +1 more source
Engineered transfer RNAs for suppression of premature termination codons [PDF]
Nature Communications, 2018 ABSTRACTPremature termination codons (PTCs) are responsible for 10-15% of all inherited disease. PTC suppression during translation offers a promising approach to treat a variety of genetic disorders, yet small molecules that promote PTC read-through have yielded mixed performance in clinical trials.
John D. Lueck +10 more
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Premature termination codons enhance mRNA decapping in human cells [PDF]
Nucleic Acids Research, 2004 Nonsense-mediated mRNA decay (NMD) is a eukaryotic surveillance process that promotes selective degradation of imperfect messages containing premature translation termination codons (PTCs). In yeast, PTCs trigger both deadenylylation-independent mRNA decapping, thereby allowing their rapid degradation by a 5' to 3' exonuclease, and to a smaller extent ...
P, Couttet, T, Grange
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How protein reads the stop codon and terminates translation [PDF]
Genes to Cells, 1998 Translation termination requires two codon‐specific protein‐release factors in prokaryotes and one factor in eukaryotes. The underlying mechanism for stop codon recognition, as well as the biological meaning of the conservation of one or two release factors in the evolutionary kingdoms, are not known.
Y, Nakamura, K, Ito
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