Results 111 to 120 of about 7,015 (221)

Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos

, 2013
When holo-aequorin is injected into zebrafish embryos at the one-cell stage, it is normally depleted by ~24 h post-fertilization (hpf). In order to acquire Ca2+ signaling information from embryos older than 24 hpf, we have developed a protocol to express
Webb, Sarah E., Chan, Ching Man, Miller, Andrew L.   +2 more
core   +1 more source

Characterization of Coelenterazine Analogs for Measurements of Luciferase Activity in Live Cells and Living Animals

Molecular Imaging, 2004
In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells.
Hui Zhao, Timothy C. Doyle, Ronald J. Wong, Yuan Cao, David K. Stevenson, David Piwnica-Worms, Christopher H. Contag   +6 more
doaj   +1 more source

Add Coelenterazine-h v1

AddCoelenterazine-has the substrate (light-sensitive; prepare fresh on the day of use) to activate the full-length RenG luciferase and measure its maximum luminescence value. This serves as the relative standard for subsequent SLCA interaction signals.
openaire   +1 more source

Coelenterazine (marine bioluminescent substrate): a source of inspiration for the discovery of novel antioxidants.

, 2005
Coelenterazine and derivatives were initially considered in the scientific community for their (bio)luminescent properties. Now, another interest of such hetero-bicycles has been pointed out by the discovery of remarkable antioxidative properties, and an
Dubuisson, Marlène, Rees, Jean-François, Marchand-Brynaert, Jacqueline   +2 more
core   +1 more source

Tumor-targeting bioluminescent bacteria for in vivo imaging

Engineering Microbiology
As the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy.
Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang   +6 more
doaj   +1 more source

Coelenterazine chemiluminescence is dependent upon superoxide anion concentrations in vitro.

, 2016
(A) HeLa cells were treated with range of 0 units/mL to 300 units/mL of PEG-SOD and imaged sequentially. The image was taken at 95 minutes post PEG-SOD addition.
Laura L. Bronsart (844933), Christian Stokes (844934), Christopher H. Contag (219505)   +2 more
core   +1 more source

Atomistic Insights into Photoprotein Formation: Computational Prediction of the Properties of Coelenterazine and Oxygen Binding in Obelin

, 2019
Bioluminescence in marine systems is dominated by the use of coelenterazine for light production. The bioluminescent reaction of coelenterazine is an enzyme catalyzed oxidative decarboxylation: coelenterazine reacts with molecular oxygen to form carbon ...
Haibo Yu (19586578), Aaron Oakley (19584748), Thomas Griffiths (19585060)   +2 more
core  

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging

Scientific Reports
Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin   +9 more
doaj   +1 more source

Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells

EMBO Reports
The p16-3MR mouse model, designed to express Renilla luciferase, mRFP, and herpes simplex virus 1 thymidine kinase (HSV-TK) under the p16 INK4a promoter, has been widely used to visualize and ablate senescent cells in vivo, but our analyses revealed ...
Nozomi Hori, Shimpei Kawamoto, Ken Uemura, Yumi Kinugasa-Katayama, Yumiko Okumura, Kentaro Tanaka, Jeong Hoon Park, Masahiro Wakita, Daisuke Motooka, Naoko Ohtani, Eiji Hara   +10 more
doaj   +1 more source