Results 101 to 110 of about 7,015 (221)

Coelenterazine detection in five myctophid species from the Kerguelen Plateau

, 2019
Myctophidae comprise the largest biomass of mesopelagic fishes in the Southern Ocean. This family has the ability to emit light – bioluminescence – via specific organs called photophores, and from the head and caudal organs. Through biochemical analyses,
Hermans, Claire, Duchatelet, Laurent, Guinet, Christophe, The Kerguelen Plateau : Marine Ecosystem + Fisheries : Proceedings of the Second Symposium, Mallefet, Jérôme, Duhamel, Guy, Cherel, Yves   +6 more
core   +1 more source

Genetically Modified Coelenterazine-Dependent Luciferases as Reporters for In Vitro Assay [PDF]

Журнал Сибирского федерального университета: Серия Биология, 2017
Among the most important tasks of modern biotechnology is the development of new analytical techniques suitable for highly sensitive and specific detection of diagnostically important targets rapidly expanding their range with the progress in biomedical ...
Ludmila A. Frank, Eugenia E. Bashmakova, Vasilisa V. Krasitskaya, Alexander N. Kudryavtsev   +3 more
doaj  

New bioluminescent coelenterazine derivatives with various C-6 substitutions

, 2017
A series of new coelenterazine analogs with varying substituents at the C-6 position of the imidazopyrazinone core have been designed and synthesized for the extension of bioluminescence substrates.
Tianyu Jiang, Lupei Du, Yubin Zhou, Ilia Yampolsky, Xingye Yang, Minyong Li   +5 more
core   +1 more source

A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level

Nature Communications
Epilepsy affects 1% of the general population and 30% of patients are resistant to antiepileptic drugs. Although optogenetics is an efficient antiepileptic strategy, the difficulty of illuminating deep brain areas poses translational challenges.
Assunta Merolla, Caterina Michetti, Matteo Moschetta, Francesca Vacca, Lorenzo Ciano, Laura Emionite, Simonetta Astigiano, Alessandra Romei, Simone Horenkamp, Ken Berglund, Robert E. Gross, Fabrizia Cesca, Elisabetta Colombo, Fabio Benfenati   +13 more
doaj   +1 more source

Solubilizing Coelenterazine in Water with Hydroxypropyl-β-cyclodextrin

Bioscience, Biotechnology, and Biochemistry, 1997
Coelenterazine is poorly soluble in water except at alkaline pH values that promote auto-oxidation. The solubility is markedly increased by adding hydroxypropyl-β-cyclodextrin in a neutral buffer solution, the solubilization being most effective in the pH range of 6.5 to 7.0.
TERANISHI, Katsunori, SHIMOMURA, Osamu
openaire   +1 more source

Highly bright and stable NIR-BRET with blue-shifted coelenterazine derivatives for deep-tissue imaging of molecular events in vivo

Theranostics, 2019
Background: Bioluminescence imaging (BLI) is one of the most widely used optical platforms in molecular imaging, but it suffers from severe tissue attenuation and autoluminescence in vivo.
Ryo Nishihara, R. Paulmurugan, Takahiro Nakajima, Eiji Yamamoto, A. Natarajan, Rayhaneh Afjei, Y. Hiruta, N. Iwasawa, S. Nishiyama, D. Citterio, Moritoshi Sato, Sung Bae Kim, Koji Suzuki   +12 more
semanticscholar   +1 more source

Self-illuminating quantum dots for highly sensitive in vivo real-time luminescent mapping of sentinel lymph nodes

International Journal of Nanomedicine, 2012
Qiang Wu, Maoquan ChuSchool of Life Science and Technology, Tongji University, Shanghai, People's Republic of ChinaAbstract: Quantum dots (QDs) show promise as novel nanomaterials for sentinel lymph node (SLN) mapping through their use in ...
Wu Q, Chu M
doaj  

The BioLuminescent-OptoGenetic in vivo Response to Coelenterazine is Proportional, Sensitive and Specific in Neocortex

bioRxiv, 2019
BioLuminescent (BL) light production can modulate neural activity and behavior through coexpressed OptoGenetic (OG) elements, an approach termed ‘BL-OG’.
M. Gomez-Ramirez, A. I. More, Nina G. Friedman, U. Hochgeschwender, C. Moore   +4 more
semanticscholar   +1 more source

Design, Synthesis, and Evaluation of the Transition- State Inhibitors of Coelenterazine Bioluminescence:  Probing the Chiral Environment of Active Site

, 2016
Design, Synthesis, and Evaluation of the Transition- State Inhibitors of Coelenterazine Bioluminescence:  Probing the Chiral Environment of Active ...
Hideshi Nakamura (2675842), Chun Wu (237024), Satoshi Inouye (203922), Akio Murai (2984127)   +3 more
core   +2 more sources

Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: Catalytic properties for the oxidation of coelenterazine as an oxygenase

, 2006
Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a complex of Ca2+-bound apoaequorin and coelenteramide, and shows luminescence activity like a luciferase, catalyzing the oxidation of coelenterazine with molecular ...
Sasaki, Satoko, Inouye, Satoshi
core   +1 more source