Results 91 to 100 of about 6,471 (200)

Design, Synthesis, and Evaluation of the Transition- State Inhibitors of Coelenterazine Bioluminescence:  Probing the Chiral Environment of Active Site

, 2016
Design, Synthesis, and Evaluation of the Transition- State Inhibitors of Coelenterazine Bioluminescence:  Probing the Chiral Environment of Active ...
Hideshi Nakamura (2675842), Chun Wu (237024), Satoshi Inouye (203922), Akio Murai (2984127)   +3 more
core   +2 more sources

Tumor-targeting bioluminescent bacteria for in vivo imaging

Engineering Microbiology
As the understanding of bacteria-mediated cancer therapies deepens, bacteria such as Escherichia coli Nissle 1917 (EcN) have become a promising platform for cancer therapy.
Chenghao Ma, Jingxi Liu, Hongjing Liu, Xiaohan Zhao, Geng Li, Youming Zhang, Tianyu Jiang   +6 more
doaj   +1 more source

Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos

, 2013
When holo-aequorin is injected into zebrafish embryos at the one-cell stage, it is normally depleted by ~24 h post-fertilization (hpf). In order to acquire Ca2+ signaling information from embryos older than 24 hpf, we have developed a protocol to express
Webb, Sarah E., Chan, Ching Man, Miller, Andrew L.   +2 more
core   +1 more source

Optimized in vivo multispectral bioluminescent imaging of tumor biology using engineered BRET reporters

iScience
Summary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra, Kshitij Parag-Sharma, John J. Powers, Sonal Srivastava, Joel R. Walker, Thomas A. Kirkland, Caroline K. Brennan, Jennifer A. Prescher, Antonio L. Amelio   +8 more
doaj   +1 more source

Dehydrogenation of coelenterazine to dehydrocoelenterazine using molecular sieves

Bioscience, Biotechnology, and Biochemistry
Abstract Coelenterazine (CTZ) and dehydrocoelenterazine (dCTZ, a dehydrogenated form of CTZ) were first identified in the liver of the luminescent squid Watasenia scintillans. In this report, we demonstrate for the first time that CTZ can be successfully converted to dCTZ using molecular sieves (MS4A/Na+, MS13X/Na+, MS5A/Ca2+, and MS3A/K+
Mitsuhiro Nakamura, Kazuya Nakayama, Kazuo Matsuda, Satoshi Inouye   +3 more
openaire   +2 more sources

An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging

Scientific Reports
Bioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao, Yan Wu, Connor Fitzgerald, Hui Wang, Tim Ugo, Tetsuo Uyeda, Wenhui Zhou, Yichi Su, Thomas A. Kirkland, Michael Z. Lin   +9 more
doaj   +1 more source

Coelenterazine chemiluminescence is dependent upon superoxide anion concentrations in vitro.

, 2016
(A) HeLa cells were treated with range of 0 units/mL to 300 units/mL of PEG-SOD and imaged sequentially. The image was taken at 95 minutes post PEG-SOD addition.
Laura L. Bronsart (844933), Christian Stokes (844934), Christopher H. Contag (219505)   +2 more
core   +1 more source

A brittle star is born: Ontogeny of luminous capabilities in Amphiura filiformis.

PLoS ONE
Bioluminescence is the production of visible light by living organisms thanks to a chemical reaction, implying the oxidation of a substrate called luciferin catalyzed by an enzyme, the luciferase.
Constance Coubris, Laurent Duchatelet, Sam Dupont, Jérôme Mallefet   +3 more
doaj   +1 more source

Coelenterazine (marine bioluminescent substrate): a source of inspiration for the discovery of novel antioxidants.

, 2005
Coelenterazine and derivatives were initially considered in the scientific community for their (bio)luminescent properties. Now, another interest of such hetero-bicycles has been pointed out by the discovery of remarkable antioxidative properties, and an
Dubuisson, Marlène, Rees, Jean-François, Marchand-Brynaert, Jacqueline   +2 more
core   +1 more source

Bioluminescent properties of obelin and aequorin with novel coelenterazine analogues.

, 2014
International audienceThe main analytical use of Ca(2+)-regulated photoproteins from luminous coelenterates is for real-time non-invasive visualization of intracellular calcium concentration ([Ca(2+)]i) dynamics in cells and whole organisms. A limitation
Borgdorff, Aren J, Vysotski, Eugene S, Picaud, Sandrine, Malikova, Natalia P, Brûlet, Philippe, Dodd, Robert H, Gealageas, Ronan, Burakova, Ludmila P   +7 more
core   +1 more source