Results 91 to 100 of about 7,015 (221)

Bioluminescence Profiling of NanoKAZ/NanoLuc Luciferase Using a Chemical Library of Coelenterazine Analogues.

Chemistry, 2019
We describe here an extensive structure-bioluminescence relationship study of a chemical library of analogues of coelenterazine, using nanoKAZ/NanoLuc, a mutated luciferase originated from the catalytic subunit of the deep-sea shrimp Oplophorus ...
Eloi P. Coutant, Glwadys Gagnot, Vincent Hervin, Racha Baatallah, S. Goyard, Y. Jacob, T. Rose, Y. Janin   +7 more
semanticscholar   +1 more source

Bioluminescent Mammalian Cells Grown in Sponge Matrices to Monitor Immune Rejection

Molecular Imaging, 2007
The growth and bioluminescence of cells seeded in collagen and gelatin sponge matrices were compared in vitro under different conditions, and immune rejection was quantified and visualized directly in situ based on loss of bioluminescence activity ...
Okechukwu Ojogho, Alice Li, Craig W. Zuppan, Alan Escher   +3 more
doaj   +1 more source

Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae

Bio-Protocol, 2017
The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain.
Steven Knafo, Andrew Prendergast, Olivier Thouvenin, Sophie Figueiredo, Claire Wyart   +4 more
doaj   +1 more source

Insect Olfactory Receptor‐Expressing Sensor Cells for Colorectal Cancer Diagnosis Using Urine Samples

Cancer Medicine, Volume 15, Issue 4, April 2026.
We developed a novel, noninvasive diagnostic method for colorectal cancer using sensor cells expressing insect ORs that detect volatile organic compounds in urine samples. By analyzing the luminescence responses of sensor cells and applying machine learning, our model achieved 80% sensitivity and an ROC‐AUC of 0.84 in distinguishing colorectal cancer ...
Koji Ando, Hiro Hasegawa, Sotaro Takiguchi, Yoshino Tonooka, Keisuke Sakasai, Michiaki Arita, Michiru Hashimoto, Chiaki Yamamoto, Manabu Toyoshima, Kentaro Kobayashi, Yasuhiko Takahashi, Masaaki Ito   +11 more
wiley   +1 more source

Coelenterazine chemiluminescence correlates with a loss of functional beta-cell mass in the MIP-Luc-VU.

, 2016
(A) Schematic representation of the study design. (B) Blood glucose concentrations of vehicle control (white) and streptozotocin (black) treated mice (n = 4). (C) Images of a representative control and streptozotocin mouse imaged with coelenterazine. (D)
Laura L. Bronsart (844933), Christian Stokes (844934), Christopher H. Contag (219505)   +2 more
core   +1 more source

Functional divergence of protein kinase A regulatory subunit Iβ variants: the importance of N3A motifs in PKA regulation

The FEBS Journal, Volume 293, Issue 8, Page 2417-2434, April 2026.
The regulatory subunit RIβ of protein kinase A occurs in two variants that differ at residue 268 (A268 versus R268). This seemingly small substitution significantly changes cAMP sensitivity, basal kinase activity, and the dynamic behavior of the regulatory domains.
Maximilian Wallbott, Jui‐Hung Weng, Valeria Pane, Yuliang Ma, Jian Wu, Susan S. Taylor, Friedrich W. Herberg   +6 more
wiley   +1 more source

Data‐Driven Feedback Identifies Focused Ultrasound Exposure Regimens for Improved Nanotheranostic Targeting of the Brain

Advanced Science, Volume 13, Issue 13, 3 March 2026.
Machine learning models predict in real time the onset of harmful microbubble collapse during microbubble‐enhanced focused ultrasound (MB‐FUS) and enable dynamic adjustment of sonication to prevent cavitation‐induced damage. This predictive control expands the safe operating window for bloodbrain barrier opening, enhancing nanoparticle delivery and ...
Hohyun Lee, Victor Menezes, Shiqin Zeng, Chulyong Kim, Cynthia M. Baseman, Jae Hyun Kim, Samhita Padmanabhan, Pranav Premdas, Naima Djeddar, Anton Bryksin, Nikhil Pandey, Pavlos Anastasiadis, Anthony J. Kim, Tobey J. MacDonald, Chetan Bettegowda, Graeme F. Woodworth, Felix J. Herrmann, Costas Arvanitis   +17 more
wiley   +1 more source

Membrane permeability of coelenterazine analogues measured with fish eggs [PDF]

Biochemical Journal, 1997
To determine the suitability of various coelenterazine analogues for the regeneration of aequorin in living cells, the membrane permeabilities of 11 analogues were measured using the eggs of the killifish Fundulus grandis by soaking the eggs in solutions containing the analogues.
openaire   +2 more sources

ATP-Binding Cassette Transporters Modulate Both Coelenterazine- and D-Luciferin-Based Bioluminescence Imaging

Molecular Imaging, 2011
Bioluminescence imaging (BLI) of luciferase reporters provides a cost-effective and sensitive means to image biological processes. However, transport of luciferase substrates across the cell membrane does affect BLI readout intensity from intact living ...
Ruimin Huang, Jelena Vider, Inna Serganova, Ronald G. Blasberg   +3 more
doaj   +1 more source

The intrinsic fluorescence of apo-obelin and apo-aequorin and use of its quenching to characterize coelenterazine binding.

, 2009
The intrinsic fluorescence of two apo-photoproteins has been characterized and its concentration-dependent quenching by coelenterazine has been for the first time applied to determine the apparent dissociation constants for coelenterazine binding with ...
Markova, S.V., Westphal, Adrie H., Eremeeva, Elena V., Berkel, Willem J.H. van, Adrie H. Westphal, Visser, Antonie J.W.G., Elena V. Eremeeva, Svetlana V. Markova, Westphal, A.H., van Berkel, W.J.H., Berkel, W.J.H., van, Eugene S. Vysotski, Eremeeva, E., Visser, A.J.W.G., Vysotski, Eugene S., Vysotski, S., Willem J.H. van Berkel, Antonie J.W.G. Visser, Markova, Svetlana V.   +18 more
core   +1 more source