Results 71 to 80 of about 7,015 (221)

Luminescence activities of reverse mutants of nanoKAZ using coelenterazine (CTZ) and CTZ analogs as a substrate.

, 2022
Luminescence activities of reverse mutants of nanoKAZ using coelenterazine (CTZ) and CTZ analogs as a substrate.
Mikako Shirouzu (272392), Yuto Sumida (1696852), Jun-ichi Sato (133357), Shun-ichi Sekine (1372875), Yuiko Sahara-Miura (1696846), Yuri Tomabechi (10644646), Satoshi Inouye (203922), Takamitsu Hosoya (205623)   +7 more
core   +1 more source

Nanodiamond Quantum Sensors for Probing Free Radical Biology

Advanced Functional Materials, Volume 36, Issue 45, 5 June 2026.
Free radicals play key roles in cellular signaling and disease but remain difficult to measure in living systems. Nanodiamonds (NDs) with nitrogen‐vacancy (NV) centers enable quantum sensing of local magnetic noise via T₁ relaxometry, providing nondestructive radical detection in living cells.
Qi Lu, Yingke Wu, Tanja Weil
wiley   +1 more source

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications

International Journal of Molecular Sciences, 2020
The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase.
V. Krasitskaya, E. Bashmakova, L. Frank
semanticscholar   +1 more source

Mechanoadaptation via Myosin Cytoplasmic Redistribution Protects Circulating Tumor Cells From Shear‐induced Death During Hematogenous Dissemination

Advanced Science, Volume 13, Issue 33, 15 June 2026.
This study investigates how CTCs survive varying shear stress during hematogenous metastasis. We uncover a self‐protection mechanism, by which non‐adherent CTCs adapt to high shearing milieu through accumulated cytoplasmic myosin‐mediated disruption of myosin‐actin binding, attenuating force transmission into chromatin to protect CTCs from shear ...
Cunyu Zhang, Qianchun Wang, Keming Li, Guanshuo Hu, Ying Xin, Kai Tang, Bing Hu, Pengyu Du, Renwei Mao, Baohua Ji, Youhua Tan   +10 more
wiley   +1 more source

Evidence For De Novo Biosynthesis of Coelenterazine in the Bioluminescent Midwater Shrimp, Systellaspis Debilis C

, 1995
Coelenterazine chemiluminescence is now established as the most common chemistry responsible for bioluminescence in the sea, being found in seven phyla. However, the organisms which synthesize coelenterazine have yet to be identified.
Herring, P. J., Thomson, Catherine M., Campbell, Anthony Keith   +2 more
core   +1 more source

In Vivo Testing of Luciferase Substrate Analogs in an Orthotopic Murine Model of Human Glioblastoma

Molecular Imaging, 2006
In vivo bioluminescent imaging using cells expressing Renilla luciferase is becoming increasingly common. Hindrances to the more widespread use of Renilla luciferase are the high autoluminescence of its natural substrate, coelenterazine, in plasma; the ...
Maya Otto-Duessel, Vazgen Khankaldyyan, Ignacio Gonzalez-Gomez, Michael C. Jensen, Walter E. Laug, Michael Rosol   +5 more
doaj   +1 more source

Solution structure of Gaussia Luciferase with five disulfide bonds and identification of a putative coelenterazine binding cavity by heteronuclear NMR

Scientific Reports, 2020
Gaussia luciferase (GLuc) is a small luciferase (18.2 kDa; 168 residues) and is thus attracting much attention as a reporter protein, but the lack of structural information is hampering further application. Here, we report the first solution structure of
N. Wu, N. Kobayashi, Kengo Tsuda, S. Unzai, Tomonori Saotome, Y. Kuroda, T. Yamazaki   +6 more
semanticscholar   +1 more source

Comprehensive transduceromic profiling of NOP receptor ligands at different Gα subunits

British Journal of Pharmacology, Volume 183, Issue 12, Page 3195-3212, June 2026.
Abstract Background and Purpose The nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP) is an opioid receptor family member with distinct pharmacology and broad therapeutic potential. NOP receptors regulate functions such as nociception, stress reactivity, reward, mood, autonomic tone, immunity and sleep/wake cycle.
Davide Malfacini, Riccardo Camilotto, Michele Maritan, Erika Morrone, Stephen Afriyie Boateng, Anna Vacilotto, Salvatore Pacifico, Remo Guerrini, Nurulain T. Zaveri, Girolamo Calo'   +9 more
wiley   +1 more source

Exploring the Potential of Arginine to Increase Coelenterazine-Renilla Luciferase Affinity and Enzyme Stability: Kinetic and Molecular Dynamics Studies

The Protein Journal
Renilla luciferase catalyzes the oxidation of coelenterazine to coelenteramide and results in the emission of a photon of light. Although Renilla luciferase has various applications in biotechnology, its low thermal stability limits the development of ...
Maryam Salehian, R. Emamzadeh, M. Nazari
semanticscholar   +1 more source

Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product

Scientific Reports, 2022
Coelenterazine-v (CTZ-v), a synthetic vinylene-bridged π-extended derivative, is able to significantly alter bioluminescence spectra of different CTZ-dependent luciferases and photoproteins by shifting them towards longer wavelengths.
Pavel V. Natashin, Elena V. Eremeeva, Mikhail B. Shevtsov, Margarita I. Kovaleva, Sergey S. Bukhdruker, Daria A. Dmitrieva, Dmitry V. Gulnov, Elena V. Nemtseva, Valentin I. Gordeliy, Alexey V. Mishin, Valentin I. Borshchevskiy, Eugene S. Vysotski   +11 more
doaj   +1 more source