Results 71 to 80 of about 6,471 (200)
Color-tunable bioluminescence imaging portfolio for cell imaging
The present study describes a color-tunable imaging portfolio together with twelve novel coelenterazine (CTZ) analogues. The three groups of CTZ analogues create diverse hues of bioluminescence (BL) ranging from blue to far red with marine luciferases ...
Shota Tamaki +6 more
doaj +1 more source
Let There be Light! Light as an Engine and Regulator in Synthetic Cells
Cells are out‐of‐equilibrium systems that regulate their spatiotemporal features. In biomimetic synthetic cells, light offers a unique way for energizing and regulating events in space and time. This review explores the implementation of light‐responsive tools in synthetic cells to replicate biological functions and enable new bioinspired behaviors ...
Matthew E. Allen +5 more
wiley +1 more source
Coelenterazine analogues emit red-shifted bioluminescence with NanoLuc
Synthesis and biological investigation of coelenterazine analogs that demonstrate a tunable red-shift in their bioluminescent emission with NanoLuc luciferase.
Joel R. Walker +5 more
core +1 more source
Bioluminescence Monitoring of Neuronal Activity in Freely Moving Zebrafish Larvae
The proof of concept for bioluminescence monitoring of neural activity in zebrafish with the genetically encoded calcium indicator GFP-aequorin has been previously described (Naumann et al., 2010) but challenges remain.
Steven Knafo +4 more
doaj +1 more source
Nanodiamond Quantum Sensors for Probing Free Radical Biology
Free radicals play key roles in cellular signaling and disease but remain difficult to measure in living systems. Nanodiamonds (NDs) with nitrogen‐vacancy (NV) centers enable quantum sensing of local magnetic noise via T₁ relaxometry, providing nondestructive radical detection in living cells.
Qi Lu, Yingke Wu, Tanja Weil
wiley +1 more source
Luciferase-Specific Coelenterazine Analogues for Optical Contamination-Free Bioassays
Spectral overlaps among the multiple optical readouts commonly cause optical contamination in fluorescence and bioluminescence. To tackle this issue, we created five-different lineages of coelenterazine (CTZ) analogues designed to selectively illuminate ...
Ryo Nishihara +5 more
doaj +1 more source
This study investigates how CTCs survive varying shear stress during hematogenous metastasis. We uncover a self‐protection mechanism, by which non‐adherent CTCs adapt to high shearing milieu through accumulated cytoplasmic myosin‐mediated disruption of myosin‐actin binding, attenuating force transmission into chromatin to protect CTCs from shear ...
Cunyu Zhang +10 more
wiley +1 more source
Coelenterazine (CLZn; 3, 7-dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo++ +[1 ,2-a]pyrazin-3-one), the substrate for bioluminescence reactions in many marine animals, is endowed with high antioxidant properties.
Trouet, André +5 more
core +1 more source
Blue fluorescent protein from the calcium-sensitive photoprotein aequorin: Catalytic properties for the oxidation of coelenterazine as an oxygenase [PDF]
Blue fluorescent protein from the calcium-binding photoprotein aequorin (BFP-aq) is a complex of Ca2+-bound apoaequorin and coelenteramide, and shows luminescence activity like a luciferase, catalyzing the oxidation of coelenterazine with molecular ...
Sasaki, Satoko, Inouye, Satoshi
core +1 more source
Cascade for conversion of coelenterazine sulfate to coelenteramide with concomitant emission of light utilizing PAP as the sulfate acceptor.
Ira Schildkraut (29742) +7 more
core +1 more source

