Results 61 to 70 of about 5,761 (165)
A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level
Nature CommunicationsEpilepsy affects 1% of the general population and 30% of patients are resistant to antiepileptic drugs. Although optogenetics is an efficient antiepileptic strategy, the difficulty of illuminating deep brain areas poses translational challenges.
Assunta Merolla +13 more
doaj +1 more source
International Journal of Nanomedicine, 2012 Qiang Wu, Maoquan ChuSchool of Life Science and Technology, Tongji University, Shanghai, People's Republic of ChinaAbstract: Quantum dots (QDs) show promise as novel nanomaterials for sentinel lymph node (SLN) mapping through their use in ...
Wu Q, Chu M
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Development of cell-impermeable coelenterazine derivatives
Chemical Science, 2013 We describe the development of the first cell-membrane impermeable coelenterazine derivative (CoelPhos). CoelPhos was constructed by the alkylation of coelenterazine with a linker containing a terminal anionic phosphonate moiety. The bioluminescence activity of CoelPhos with Gaussia luciferase (GLuc) showed a significantly higher activity in comparison
Eric Lindberg +5 more
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Molecular Imaging, 2004 In vivo imaging of bioluminescent reporters relies on expression of light-emitting enzymes, luciferases, and delivery of chemical substrates to expressing cells.
Hui Zhao +6 more
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iScienceSummary: The ability to visualize and track multiple biological processes in vivo in real time is highly desirable. Bioluminescence imaging (BLI) has emerged as an attractive modality for non-invasive cell tracking, with various luciferase reporters ...
Bryan Labra +8 more
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An optimized luciferin formulation for NanoLuc-based in vivo bioluminescence imaging
Scientific ReportsBioluminescence imaging (BLI) is widely used in preclinical biomedical research for noninvasive tracking of cell populations and biochemical events in vivo.
Chao Gao +9 more
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Limitations of the p16-3MR mouse model for detecting and eliminating senescent cells
EMBO ReportsThe p16-3MR mouse model, designed to express Renilla luciferase, mRFP, and herpes simplex virus 1 thymidine kinase (HSV-TK) under the p16 INK4a promoter, has been widely used to visualize and ablate senescent cells in vivo, but our analyses revealed ...
Nozomi Hori +10 more
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AddCoelenterazine-has the substrate (light-sensitive; prepare fresh on the day of use) to activate the full-length RenG luciferase and measure its maximum luminescence value. This serves as the relative standard for subsequent SLCA interaction signals.
openaire +1 more source
Biochemistry Research InternationalBioluminescence has been a fascinating natural phenomenon of light emission from living creatures. It happens when the enzyme luciferase facilitates the oxidation of luciferin, resulting in the creation of an excited-state species that emits light ...
Asiri N. Dunuweera +2 more
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Iranian South Medical Journal, 2015 Background: Aequorin is a calcium sensitive photoprotein composed of apoaequorin (189 amino acid), oxygen and coelenterazine (imidazopyrazine chromophore). Activity of this photoprotein in calcium presence is blue photon emission (469 nm).
Faeazeh Ghanbartalab +5 more
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