Results 91 to 100 of about 418,378 (371)

The IFNγ‐CIITA‐MHC II axis modulates melanoma cell susceptibility to NK‐cell‐mediated cytotoxicity

Molecular Oncology, EarlyView.
Natural killer (NK) cells play a central role in anti‐melanoma immunity. However, melanoma cells adapt during co‐culture by upregulating CIITA and MHC II in response to interferon gamma (IFNγ), thereby evading NK‐cell lysis. Blocking IFNγ signaling or treatment with dimethyl fumarate/simvastatin counteracts this immune escape and maintains NK‐cell ...
Lena C. M. Krause, Rixa‐Mareike Köhn, Christian Ickes, Julia Lenger, Jonas Fischer, Sabrina Cappello, Ivan Bogeski   +6 more
wiley   +1 more source

Tubulin cofactors and Arl2 are cage-like chaperones that regulate the soluble αβ-tubulin pool for microtubule dynamics

eLife, 2015
Microtubule dynamics and polarity stem from the polymerization of αβ-tubulin heterodimers. Five conserved tubulin cofactors/chaperones and the Arl2 GTPase regulate α- and β-tubulin assembly into heterodimers and maintain the soluble tubulin pool in the ...
Stanley Nithianantham, Sinh Le, Elbert Seto, Weitao Jia, Julie Leary, Kevin D Corbett, Jeffrey K Moore, Jawdat Al-Bassam   +7 more
doaj   +1 more source

New cofactor supports α,β-unsaturated acid decarboxylation via 1,3-dipolar cycloaddition

Nature, 2015
The bacterial ubiD and ubiX or the homologous fungal fdc1 and pad1 genes have been implicated in the non-oxidative reversible decarboxylation of aromatic substrates, and play a pivotal role in bacterial ubiquinone (also known as coenzyme Q) biosynthesis ...
K. Payne, M. White, K. Fisher, B. Khara, S. Bailey, David Parker, N. Rattray, D. Trivedi, R. Goodacre, R. Beveridge, P. Barran, S. Rigby, N. Scrutton, Sam Hay, D. Leys   +14 more
semanticscholar   +1 more source

Intein‐based modular chimeric antigen receptor platform for specific CD19/CD20 co‐targeting

Molecular Oncology, EarlyView.
CARtein is a modular CAR platform that uses split inteins to splice antigen‐recognition modules onto a universal signaling backbone, enabling precise, scarless assembly without re‐engineering signaling domains. Deployed here against CD19 and CD20 in B‐cell malignancies, the design supports flexible multi‐antigen targeting to boost T‐cell activation and
Pablo Gonzalez‐Garcia, Noelia Moares, Wenjie Yi‐He, Rosa Luna‐Espejo, Ricardo Fernandez‐Cisnal, Javier Ocaña‐Cuesta, Juan P. Muñoz‐Miranda, Antonio Gabucio, Cecilia M. Fernandez‐Ponce, Francisco Garcia‐Cozar   +9 more
wiley   +1 more source

Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding

Nature Communications, 2016
Fdc1 is a decarboxylase enzyme that requires the novel prenylated FMN cofactor for activity. Here, we use it as an exemplar system to show how native top-down and bottom-up mass spectrometry can measure the structural effect of cofactor binding by a ...
R. Beveridge, Lukasz G. Migas, K. Payne, N. Scrutton, D. Leys, P. Barran   +5 more
semanticscholar   +1 more source

Two‐way inhibition of PAX5 transcriptional activity by PAX5::CBFA2T3

FEBS Open Bio, EarlyView.
PAX5::CBFA2T3 (PAX5‐C) is a fusion protein of the B‐cell transcription factor, PAX5, and is found in B‐cell ALL. We propose a putative model of two‐way inhibition of PAX5 transcriptional activity by PAX5‐C. There are two ways of repression by PAX5‐C: DNA‐binding‐dependent way and HDAC‐dependent way, with either being sufficient for the repression. HDAC
Reina Ueno, Aki Terasaki, Yuiko Imai, Yuri Kimura, Yuna Kojima, Misa Irie, Koya Odaira, Mina Noura, Shuichi Okamoto, Takahiko Yasuda, Shinobu Tsuzuki, Hitoshi Kiyoi, Fumihiko Hayakawa   +12 more
wiley   +1 more source

Increasing 1,4-Diaminobutane Production in Escherichia coli by Optimization of Cofactor PLP and NADPH Synthesis

Molecules
1,4-diaminobutane is widely used in the industrial production of polymers, pharmaceuticals, agrochemicals and surfactants. Owing to economic and environmental concerns, there has been a growing interest in using microbes to produce 1,4-diaminobutane ...
Tong Sun, Yongcan Zhao, Jinjin Wang, Wenke Kang, Xiangxiang Sun, Yanling Sun, Meixue Chu, Zhengyu Liu, Fuping Lu, Ming Li   +9 more
doaj   +1 more source

Structural and functional analysis of Bacillus sarcosine oxidase and its activity toward cyclic imino acids

FEBS Open Bio, EarlyView.
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang, Yoshitaka Nakajima, Masae Kurobe, Tsutomu Nakamura, Tomoki Himiyama, Yoshiaki Nishiya   +5 more
wiley   +1 more source

Multicenter validation of an RNA-based assay to predict anti-PD-1 disease control in patients with recurrent or metastatic head and neck squamous cell carcinoma: the PREDAPT study

Journal for ImmunoTherapy of Cancer
Background Despite advances in cancer care and detection, >65% of patients with squamous cell cancer of the head and neck (HNSCC) will develop recurrent and/or metastatic disease. The prognosis for these patients is poor with a 5-year overall survival
Howard L McLeod, A Dimitrios Colevas, Georges Azzi, Kevin C Flanagan, Douglas Adkins, Jessica Ley, Ezra E W Cohen, James Wade, Jon Earls, Jeffrey Hiken, Rachel L Wellinghoff, Michelle M Ponder, William H Westra, Vera Vavinskaya, Leisa Sutton, Ida Deichaite, Orlan K Macdonald, Karim Welaya, Andrew W Pippas, Jennifer Slim, Bruce Bank, Xingwei Sui, Steven E Kossman, Todd D Shenkenberg, Warren L Alexander, Katharine A Price, David N Messina, Jarret I Glasscock, Eric J Duncavage   +28 more
doaj   +1 more source

Overview on the Bacterial Iron-Riboflavin Metabolic Axis

Frontiers in Microbiology, 2018
Redox reactions are ubiquitous in biological processes. Enzymes involved in redox metabolism often use cofactors in order to facilitate electron-transfer reactions. Common redox cofactors include micronutrients such as vitamins and metals.
Ignacio Sepúlveda Cisternas, Juan C. Salazar, Víctor A. García-Angulo   +2 more
doaj   +1 more source