Results 241 to 250 of about 418,378 (371)

MnO2 Nanosponge‐Accelerated Cas12a Trans‐Cleavage: Breaking the Kinetic Barrier for In Vivo RNA Imaging

open access: yesAdvanced Science, EarlyView.
A Cas12a@MnO2 nanosponge (hMNS) nanoprobe is engineered to overcome the intracellular delivery and kinetic barrier of the conventional CRISPR/Cas12a system via GSH‐responsive release and Mn2⁺‐enhanced trans‐cleavage. It enables preamplification‐free detection of mRNA with single‐cell sensitivity, differentiation of mRNA expression between breast cancer
Wen‐jing Liu   +3 more
wiley   +1 more source

On the cofactor of glucose dehydrogenase of Pseudomonas fluorescens.

open access: bronze, 1979
Yujiro Imanaga   +4 more
openalex   +2 more sources

Control of p97 function by cofactor binding

open access: yesFEBS Letters, 2015
A. Buchberger   +2 more
semanticscholar   +1 more source

Distinct Phage‐Encoded Enzymes for Substitution of Deoxythymidine by Deoxyuridine in Phage Genomes

open access: yesAdvanced Science, EarlyView.
Distinct enzymes including dCTP deaminases (Dcds), dTMP phosphatases (Dtms), and dTTP pyrophosphatases (Dtt) are found to be responsible for dU‐DNA biosynthesis in the phages PBS1, DSS3_VP1, and PhiR1‐37. Both PhiR1‐37‐derived and synthetic dU‐DNA are resistant to cleavage by restriction enzymes and Cas12a nuclease targeting dT‐containing sites.
Yating Li   +7 more
wiley   +1 more source

Anticardiolipin antibodies (ACA) directed not to cardiolipin but to a plasma protein cofactor

open access: yesThe Lancet, 1990
M. Galli   +8 more
semanticscholar   +1 more source

BRIGHT Enables High‐SNR Live‐Cell Imaging of Non‐Repetitive Sequences via Bivalent Fluorescent Nanobody‐Mediated Cascade‐Dependent Illumination

open access: yesAdvanced Science, EarlyView.
BRIGHT is a newly developed live‐cell imaging system that integrates cascade amplification with background denoising through the bivalent binding capability and antigen‐dependent stability of a bivalent fluorescent nanobody targeting ALFA peptides.
Lei Feng   +9 more
wiley   +1 more source

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