A CALB-like Cold-Active Lipolytic Enzyme from Pseudonocardia antarctica: Expression, Biochemical Characterization, and AlphaFold-Guided Dynamics [PDF]
Marine DrugsCold-active lipolytic enzymes enable low-temperature biocatalysis, but remain underexplored in Antarctic actinomycetes. Here, we report the discovery and first-step characterization of a CALB-like cold-active lipolytic enzyme (PanLip) from Pseudonocardia
Lixiao Liu +4 more
doaj +2 more sources
Transcriptomic profiling reveals stage-specific responses to cold stress in rice seedlings [PDF]
BMC Plant BiologyCold stress severely restricts rice growth, development, productivity, and geographical distribution. Here, we performed dynamic transcriptome profiling to investigate cold tolerance and cold-responsive metabolic pathways across distinct seedling growth ...
Yanting Li +11 more
doaj +2 more sources
Engineering a Cold-Active Cellulase Complex with a Novel Mushroom Cellobiohydrolase for Efficient Biomass Saccharification and Juice Flavor Optimization [PDF]
Journal of FungiCold-active cellulases are highly desirable for temperature-sensitive biomass valorization and food processing, yet they remain scarce in conventional industrial fungal platforms.
Jiaqi Yang +9 more
doaj +2 more sources
PLoS ONE, 2023 In this work, a psychrotrophic bacteria producing cold-active protease, was obtained from Dachigam National Park, an ecologically significant habitat in Western Himalayas owing to its varied endemic and endangered flora and fauna.
Hina Mushtaq +4 more
doaj +2 more sources
Highly Stable, Cold-Active Aldehyde Dehydrogenase from the Marine Antarctic Flavobacterium sp. PL002
Fermentation, 2021 Stable aldehyde dehydrogenases (ALDH) from extremophilic microorganisms constitute efficient catalysts in biotechnologies. In search of active ALDHs at low temperatures and of these enzymes from cold-adapted microorganisms, we cloned and characterized a ...
Georgiana Necula-Petrareanu +5 more
doaj +1 more source
Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4 Å resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme. [PDF]
PLoS ONE, 2018 Enzymes isolated from organisms found in cold habitats generally exhibit higher catalytic activity at low temperatures than their mesophilic homologs and are therefore known as cold-active enzymes.
Ha Ju Park +8 more
doaj +1 more source
Endolysins from Antarctic Pseudomonas Display Lysozyme Activity at Low Temperature
Marine Drugs, 2020 Organisms specialized to thrive in cold environments (so-called psychrophiles) produce enzymes with the remarkable ability to catalyze chemical reactions at low temperature.
Marco Orlando +2 more
doaj +1 more source
Trehalose An Additive Solution for Platelet Concentrate to Protect Platelets from Apoptosis and Clearance during Their Storage at 4°C [PDF]
Cell Journal, 2022 Objective: Although cold storage of platelets (PLTs) could decrease the risk of bacterial growth, it could affect on the PLTs viability and hemostatic function.
Vahid Baghdadi +3 more
doaj +1 more source
α-Glucosidase and β-glucosidase from psychrotrophic strain arthrobacter sp. C2-2
Czech Journal of Food Sciences, 2005 In this work six psychophilic and psychrotrophic bacterial strains were screened for the presence of different glycosidase activities (α-galactosidase, α-glucosidase, β-glucosidase, α-mannosidase and β-glucuronidase).
Eva Benešová +2 more
doaj +1 more source
Systematic investigation of the link between enzyme catalysis and cold adaptation
eLife, 2022 Cold temperature is prevalent across the biosphere and slows the rates of chemical reactions. Increased catalysis has been predicted to be a dominant adaptive trait of enzymes to reduced temperature, and this expectation has informed physical models for ...
Catherine Stark +3 more
doaj +1 more source