Results 81 to 90 of about 5,544,180 (301)

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Energy expenditure, body core temperature, mean skin temperature and core-skin temperature gradient in thermoneutral conditions (TN) and during mild cold (Cold) in morbidly obese subjects.

, 2013
Energy expenditure, body core temperature, mean skin temperature and core-skin temperature gradient in thermoneutral conditions (TN) and during mild cold (Cold) in morbidly obese subjects.
G. J. Jaap Teule (355643), Boudewijn Brans (355645), Patrick Schrauwen (42320), Wouter D. van Marken Lichtenbelt (42321), Nicole D. Bouvy (355641), Guy H. E. J. Vijgen (355640)   +5 more
core   +1 more source

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

NASA Cold Land Processes Experiment (CLPX 2002/03): ground-based and near-surface meteorological observations [PDF]

, 2009
A short-term meteorological database has been developed for the Cold Land Processes Experiment (CLPX). This database includes meteorological observations from stations designed and deployed exclusively for CLPXas well as observations available from other
Elder, Kelly, Mahrt, L., Rutter, Nick, Goodbody, Angus, Houser, P., Cline, Don   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Preferential translation of cold-shock mRNAs during cold adaptation

, 2004
Upon temperature downshift below the lower threshold of balanced growth (∼20°C), the Escherichia coli translational apparatus undergoes modifications allowing the selective translation of the transcripts of cold shock-induced genes, while bulk protein ...
PON, Cynthia, GUALERZI, Claudio, GIULIODORI, Anna Maria, BRANDI, Anna   +3 more
core   +1 more source

Effect Of Cold On Growth Of Fungal Elements In Clinical Specimens

پیاورد سلامت, 2016
Background and Aim: In medical centers, after obtaining visceral clinical samples in suitable containers under certain circumstances, they are sent to mycology laboratory.
Ghafur Tavakoli, Roshanak Daei, Farshad Hashemi, Mehdi Zarei, Hoda Deli, Seyed Jamal Hashemi   +5 more
doaj  

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Impacts of heat, cold, and temperature variability on mortality in Australia, 2000-2009

, 2019
Objectives: Evidence is limited on the relative contribution of different temperature exposures (i.e., heat, cold and significant temperature variability) to mortality.
Su, Hong, Cheng, Jian, Bambrick, Hilary, Tong, Shilu, Hu, Wenbiao, Xu, Zhiwei   +5 more
core   +1 more source

Cold-sintered temperature stable Na0.5Bi0.5MoO4−Li2MoO4 microwave composite ceramics [PDF]

, 2017
A cold sintering process (150 °C, 30 min and 200 MPa) was employed to fabricate Na0.5Bi0.5MoO4−Li2MoO4 (NBMO-LMO) composites with up to 96.4% relative density.
Shiyu Zhang (1222824), Ian M. Reaney (1683007), Di Zhou (126052), Dawei Wang (471687), William Whittow (1224405), Darren Cadman (1250775), J. C. Vardaxoglou (1257960)   +6 more
core   +2 more sources