Results 271 to 280 of about 14,287 (299)

The genetics and physiology of coliphage N4

Virology, 1973
Amber mutants of bacteriophage N4 were isolated and classified into 27 cistrons by complementation tests. A group of 5 cistrons are essential for the synthesis of phage DNA, whereas 22 other cistrons, when defective, permit varying degrees of N4 DNA replication.
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Molecular weights of coliphages and coliphage DNA

Journal of Molecular Biology, 1970
Abstract The range of usefulness of the high-speed equilibrium centrifugation method of Yphantis (1964) has been extended to measure the molecular weight of Escherichia coli phage T7. Values for \ gn of phages T7, T5 and T4 were obtained by pycnometry; the phage concentrations were determined by measuring nitrogen and phosphorus contents, and ...
Frank C. Bancroft, David Freifelder
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The nu1 gene of coliphage λ

Virology, 1979
Abstract The phage mutant λ nu lt16, which is defective in DNA packaging, defines a new λ gene, nu 1. This has been shown by its ability to complement and be complemented by λ mutants defective in all of the other morphogenetic genes. The nu lt 16 mutation is a 1.28-kb DNA insertion located to the left of gene A , the leftmost known cistron on ...
Robert A. Weisberg, Edith Gallay, Nat Sternberg   +2 more
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In vitro initiation of coliphage T7 mRNA

Archives of Biochemistry and Biophysics, 1972
Abstract The synthesis of two classes of late T7 proteins appears to be directed by several polycistronic messenger RNAs. To understand how they are translated in appropriate order during phage infection, we have isolated late mRNA from T7-infected cells and mRNA that was synthesized in vitro with the T7 RNA polymerase and T7 DNA.
Philip Leder, Robert Callahan
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Cyclization of coliphage 186 DNA

Journal of Molecular Biology, 1967
Equilibrium and kinetic studies have been made on the cyclization of coliphage 186 DNA. In 0·13 m -Na + , the melting temperature of the cohesive ends ( T m ), i.e. the midpoint of the linear monomer to cyclic monomer transition, occurs at 63°C, which is 12°C above that of λb2b5c DNA in the same medium.
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Active Form of Two Coliphage Repressors

Nature, 1970
Two coliphage repressers were originally isolated as monomeric proteins. Here it is shown that these repressers attach to DNA in the form of oligomers, possibly as tetramers, and that these oligomers are in rapid equilibrium with the monomers.
Paul Chadwick, Mark Ptashne, Vincenzo Pirrotta   +2 more
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Coliphages as Indicators for the Microbial Quality of Treated Wastewater Effluents

Food and Environmnetal Virology, 2021
A. Nasser, S. Sasi, Y. Nitzan
semanticscholar   +1 more source

Physical map of coliphage N4 DNA

Virology, 1980
Abstract A detailed restriction endonuclease map of Escherichia coli phage N4 DNA is presented. The cleavage sites for restriction endonucleases Hae II (1 cut), Hha I (3 cuts), Xba I (5 cuts), Ava II (15 cuts), and Hpa I (25 cuts) have been determined by (1) digestion with endonucleases after end labeling of the DNA; (2) digestion with two ...
C. Malone, R. Zivin, Lucia B. Rothman-Denes   +2 more
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Inactivation of the T7 coliphage by the diepoxybutane stereoisomers

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
Abstract 1. 1. At any time after a 30-min treatment, the diepoxybutane stereoisomers classify in the following order of decreasing toxicities towards the T 7 coliphage; l -, d - and meso -. 2. 2. DNA interstrand crosslinks are found after a treatment with the l - or the d -isomer, but not with meso-diepoxybutane.
L. Brakier, P.W. Feit, W.G. Verly
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Fast and easy methods for the detection of coliphages.

Journal of Microbiological Methods, 2020
A. Blanch, F. Lucena, M. Muniesa, J. Jofre   +3 more
semanticscholar   +1 more source