Results 51 to 60 of about 20,337 (280)
Isolation of Adult Rat Cardiomyocytes Using Recombinant Collagenases
Chemical Engineering Transactions, 2018 Direct isolation of primary cells from tissues and organs allows for the maintenance of important cell characteristics and properties for in vitro studies and a plethora of biomedical applications.
S. Campora +3 more
doaj +1 more source
Thin-Capped Atheromata With Reduced Collagen Content in Pigs Develop in Coronary Arterial Regions Exposed to Persistently Low Endothelial Shear Stress [PDF]
, 2012 Objective—The mechanisms promoting the focal formation of rupture-prone coronary plaques in vivo remain incompletely understood. This study tested the hypothesis that coronary regions exposed to low endothelial shear stress (ESS) favor subsequent ...
Antoniadis, Antonios P. +15 more
core +1 more source
FEBS Open Bio, EarlyView.This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia +3 more
wiley +1 more source
Фармация и фармакология (Пятигорск), 2019 The aim of this study was to assess the coverage of studies of collagenolytic enzymes (collagenases) in the sphere of their use in wounds and scars treatment, the resource of their production and the range of collagenase products to identify the areas ...
A. V. Mayorova +3 more
doaj +1 more source
The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation [PDF]
Archives of Biological Sciences, 2010 Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets.
Nikolić D. +7 more
doaj +1 more source
STAR Protocols, 2023 Summary: Inter-domain movements act as important activity modulators in multi-domain proteins. Here, we present a protocol for inter-domain cross-linking via engineered cysteines.
Jamil Serwanja +2 more
doaj +1 more source
Advanced Functional Materials, EarlyView.There is a significant need for biomaterials with well‐defined stability and bioactivity to support tissue regeneration. In this study, we developed a tunable microgel platform that enables the decoupling of stiffness from porosity, thereby promoting bone regeneration.
Silvia Pravato +9 more
wiley +1 more source
Matrix metalloproteinases-2,-3,-7,-9 and-10, but not MMP-11, are differentially expressed in normal, benign tumorigenic and malignant human keratinocyte cell lines [PDF]
, 2000 In order to investigate the correlations between constitutive proteinase expression and the degree of tumorigenicity of cancer cells we have studied a model system of three keratinocyte cell lines.
Bachmeier, B. E. +4 more
core +1 more source
Synthetic Nanobiology Actuated Lipometabolic Cell Factory for Autologous Tumor Immunotherapy
Advanced Functional Materials, EarlyView.FA plays a crucial role in the interaction between tumor cells and the tumor microenvironment, especially for the immune response. A biocatalytic immunoenhancement strategy is developed to boost antitumor immunity by FA metabolic orientation to ceramide. Through the design of this delicate catalytic immunoenhancement strategy, the synthetic nanobiology
Shoujie Zhao +8 more
wiley +1 more source
Chemical Engineering Transactions, 2012 One of the main success practices in cell therapy is the transplantation of allogeneic pancreatic islets cells in patients with type 1 diabetes. However, at the moment less than 50 % of procedures are successfully completed; this is because the procedure
M. Salamone +5 more
doaj +1 more source