Results 31 to 40 of about 11,362 (251)

Therapeutic applications of collagenase (metalloproteases): A review

Asian Pacific Journal of Tropical Biomedicine, 2016
Non-invasive therapeutic methods have recently been used in medical sciences. Enzymes have shown high activity at very low concentrations in laboratories and pharmaceutical, enabling them to play crucial roles in different biological phenomena related to
Hamzeh Alipour, Abbasali Raz, Sedigheh Zakeri, Navid Dinparast Djadid   +3 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Isolation of Adult Rat Cardiomyocytes Using Recombinant Collagenases

Chemical Engineering Transactions, 2018
Direct isolation of primary cells from tissues and organs allows for the maintenance of important cell characteristics and properties for in vitro studies and a plethora of biomedical applications.
S. Campora, P. Sanjuan Alberte, C. Bruno, G. Ghersi   +3 more
doaj   +1 more source

Characterizing Cutaneous α‐Synuclein Deposition and Seeding Activity in Parkinson's Disease Subtypes

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Cutaneous phosphorylated α‐synuclein (p‐syn) and α‐synuclein seeding activity are promising biomarkers for Parkinson's disease (PD), but their clinical value remains uncertain due to disease heterogeneity. This study evaluates these two biomarkers in PD patients to inform phenotype‐specific diagnosis and disease severity assessment ...
Yuting Jin, Tianyuan Yang, Minglei Liu, Haiyang Luo, Jing Yuan, Ke Zhang, Xinsheng Han, Jing Yang, Yuming Xu   +8 more
wiley   +1 more source

Vibrio Proteases for Biomedical Applications: Modulating the Proteolytic Secretome of V. alginolyticus and V. parahaemolyticus for Improved Enzymes Production

Microorganisms, 2019
Proteolytic enzymes are of great interest for biotechnological purposes, and their large-scale production, as well as the discovery of strains producing new molecules, is a relevant issue.
Monica Salamone, Aldo Nicosia, Giulio Ghersi, Marcello Tagliavia   +3 more
doaj   +1 more source

Microenvironmental Reprogramming by 3D Anisotropic Cardiac Extracellular Matrix Induces Nuclear Remodeling and Epigenetic Maturation of Chemically Induced Cardiomyocytes

Advanced Functional Materials, EarlyView.
A 3D anisotropic hydrogel derived from heart extracellular matrix guides cytoskeletal alignment and nuclear remodeling in reprogrammed cardiomyocyte‐like cells. This study reveals how matrix alignment modulates nuclear envelope dynamics and chromatin state, triggering transcriptional and functional maturation.
Seung Ju Seo, Mi Jeong Lee, Hyun Wook Kang, Seonhee Byeon, Soo‐Kyoung Choi, Nakwon Choi, Seung‐Woo Cho, Yoonhee Jin   +7 more
wiley   +1 more source

Biochemical Comparison Between Clostridium Hystoliticum Collagenases G and H obtained by DNA Recombinant and Extractive Procedures

Chemical Engineering Transactions, 2012
One of the main success practices in cell therapy is the transplantation of allogeneic pancreatic islets cells in patients with type 1 diabetes. However, at the moment less than 50 % of procedures are successfully completed; this is because the procedure
M. Salamone, A. Cuttitta, F. Bertuzzi, C. Ricordi, G. Ghersi, G. Seidita   +5 more
doaj   +1 more source

The effect of different concentrations of Liberase HI used in a non automated method for human adult pancreatic islet isolation [PDF]

Archives of Biological Sciences, 2010
Liberase HI has many advantages over traditional collagenases. Liberase performs the rapid dissociation of human pancreata and allows for the preservation of the morphological and functional integrity of isolated islets.
Nikolić D., Đorđević P.B., Dimitrijević-Srecković Vesna, Paunović I., Kalezić Nevena, Popović S., Stefanović D., Džingalašević Marina   +7 more
doaj   +1 more source

Quantitative cross-linking via engineered cysteines to study inter-domain interactions in bacterial collagenases

STAR Protocols, 2023
Summary: Inter-domain movements act as important activity modulators in multi-domain proteins. Here, we present a protocol for inter-domain cross-linking via engineered cysteines.
Jamil Serwanja, Hans Brandstetter, Esther Schönauer   +2 more
doaj   +1 more source

Hierarchical Collagen Nanofiber Gels Recapitulate a Physiologically Relevant Tumor Stromal Microenvironment

Advanced Healthcare Materials, EarlyView.
The collagen nanofiber (CNF) gel supports cancer cell culture, exhibiting cell morphology and cancer‐related protein expression similar to Matrigel. It offers a higher collagen concentration than commercial gels. Additionally, the CNF gel allows replication of the high elasticity of in vivo cancer tissue by controlling collagen fiber sizes, ranging ...
Naoko Sasaki, Tomoyuki Suezawa, Shiro Kitano, Ryohei Katayama, Michiya Matsusaki   +4 more
wiley   +1 more source