Results 101 to 110 of about 911,219 (263)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Study on the Aging Mechanism of Textile Relics in Museum Collection Environment by Accelerated Aging Experiment

open access: yesJournal of Natural Fibers
The protection of textile relics against environment-induced aging is vital when planning exhibitions or storing in the museum. To effectively avoid the deterioration of textile relics caused by improper preservation environment, this study accordingly ...
Yuhui Wei   +7 more
doaj   +1 more source

Discrepancy in different numbers of colors

open access: yesDiscrete Mathematics, 2002
The author investigates discrepancies of hypergraphs. In particular, he determines the multi-color discrepancies of the \(k\)-balanced hypergraph \(\mathcal{H}_{nk}\) on partition classes of (equal) size \(n.\)
openaire   +2 more sources

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Hybrid Drying of Apples: A Comparison of Continuous and Intermittent Process Modes

open access: yesApplied Sciences
In recent years, microwave and ultrasound technology has been under extensive development in drying technologies. Researchers are constantly searching for improved solutions or alternatives to hot air drying.
Justyna Szadzińska   +2 more
doaj   +1 more source

Linked-color Imaging May Help Improve the Visibility of Superficial Barrett's Esophageal Adenocarcinoma by Increasing the Color Difference. [PDF]

open access: yesIntern Med, 2021
Saito M   +10 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

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