Results 91 to 100 of about 466,071 (297)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Practical application of browns and grays based on a vector concept : the practical strength the asymmetrical Munsell color space

, 2018
A comparison was done regarding tertiary students' response between the symmetrical color wheel—The Itten Color Wheel developed by Johanne Itten at Bauhaus School and the asymmetrical Munsell Color Space developed by Professor Albert Munsell.
Woon Lam Ng, Ng, Woon Lam
core   +1 more source

Development of a Prototype for the Quality Control of Bovine Meat Determined by its Organoleptic Characteristics, based on an Automatic Inspection System for Artificial Vision

Latin-American Journal of Computing, 2017
The world beef market is in full growth and with unsatisfied demands and prices up and these depend very much on their quality when arriving at the final consumer, while the national market, our country also has a future potential in the meat industry ...
Paola Portero, Bella Mena, Javier Gavilanes   +2 more
doaj  

Using a Reference Color Plate to Correct Smartphone-Derived Soil Color Measurements with Different Smartphones Under Different Lighting Conditions

Soil Systems
Soil color has long been used as an indicator for soil properties such as soil organic carbon and soil moisture. Recent developments in citizen science have seen the increased use of smartphone cameras for soil color measurements. However, there are high
Sheng Li, Fangzhou Zheng, Alexander J. Koiter, Yulia Kupriyanovich, David A. Lobb, Masoud Goharrokhi   +5 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Visually-based color space tetrahedrizations for printing with custom inks [PDF]

Printing with custom inks is useful for extending the gamut of printed images, for creating artistically appealing designs or for providing protection against counterfeiting (security documents).
Chosson, S. M., Hersch, R. D.
core   +1 more source

Using the watershed method in determining the size of intersecting particles of crushed grain

Аграрная наука Евро-Северо-Востока
The determination of the granulometric composition of ground grain is of great importance when feeding farm animals and poultry. The sieve method of determining the granulometric composition is traditionally used.
S. Yu. Bulatov, V. G. Krestinkov, G. S. Malyshev, O. A. Tareeva, A. E. Shamin   +4 more
doaj   +1 more source