Results 81 to 90 of about 466,071 (297)

Reproducible color gamut of hematoxylin and eosin stained images in standard color spaces

Journal of Pathology Informatics, 2020
A whole-slide imaging (WSI) system is a digital color imaging system used in digital pathology with the potential to substitute the conventional light microscope. A WSI system digitalizes a glass slide by converting the optical image to digital data with
Wei- Chung Cheng
doaj   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

An object-color space

Journal of Vision, 2009
Putting aside metaphorical meanings of the term, color space is understood as a vector space, where lights having the same color (i.e., subjectively indistinguishable) are represented as a point. The CIE 1931 color space, empirically based on trichromatic color measurements, is a classical example.
openaire   +3 more sources

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Ion-Selective Optical Sensors: A New Look at Well-Established Techniques of Signal Acquisition

Proceedings, 2018
It is demonstrated for the first time that the registration of the optode signal under non-equilibrium conditions reduces analysis time and shifts the sensor working range.
Andrey Kalinichev, Maria Peshkova, Nadezhda Pokhvishcheva, Konstantin Mikhelson   +3 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Influence of technology, color architecture and bit-depth of optoelectronic imaging sensors used as color measurement instruments

, 2005
Digital image capture devices cannot be used directly as instruments for color measurement. The main stages for characterizing a digital image capture device as an instrument for color measurement are the spectral and spatial characterization and the ...
Martínez-Verdú, Francisco M., Viqueira Pérez, Valentín, Perales, Esther, Fez Saiz, Dolores de, Pujol Ramo, Jaume, Perales Romero, Esther, Arjona Carbonell, Montserrat, Vilaseca Ricart, Meritxell, Viqueira, Valentín, Lasarte Rigueiro, Marta de   +9 more
core  

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Advancement in Color Image Processing using Geometric Algebra [PDF]

, 2008
This paper describes an advancement in color image processing, using geometric algebra. This is achieved using a compact representation of vectors within $n$ dimensional space. Geometric Algebra (GA) is a preferred framework for signal representation and
Mishra, Biswajit, Al-Hashimi, Bashir, Wilson, Peter   +2 more
core   +1 more source