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Journal of Diabetes Investigation, Volume 17, Issue 7, Page 1083-1086, July 2026.
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Biochemical Society Transactions, 2009 The single-cell gel electrophoresis technique or comet assay is widely regarded as a quick and reliable method of analysing DNA damage in individual cells. It has a proven track record from the fields of biomonitoring to nutritional studies. The assay operates by subjecting cells that are fixed in agarose to high salt and detergent lysis, thus removing
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The comet assay: a comprehensive review
Mutation Research - Reviews in Genetic Toxicology, 1995The comet assay is a sensitive and rapid method for DNA strand break detection in individual cells. Its use has increased significantly in the past few years. This paper is a review of the studies published to date that have made use of the comet assay.
Daryl W Fairbairn +2 more
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The Comet Assay in Marine Animals
, 2019 Comet assay is a quick and versatile technique for assessing DNA damage in individual cells. It allows for the detection of DNA single- and double-strand breaks, as well as the presence of alkali labile sites and cross-links. Here we describe protocols for the single-cell gel electrophoresis (Comet assay) in its alkaline (pH > 13), mild alkaline (pH ...
Guidi, Patrizia +2 more
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, 2014 (A) Samples were subjected to alkaline comet assay to monitor DNA damage stained with SYBR Green I and Exλ 488 nm, Emλ 520 nm. Images were capture using Olympus BX60 florescence microscope at 20x.
Solange Costa, João Paulo Teixeira
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The Comet Assay in Human Biomonitoring
2013Human biomonitoring studies aim to identify potential exposures to environmental, occupational, or lifestyle toxicants in human populations and are commonly used by public health decision makers to predict disease risk. The Comet assay measures changes in genomic stability and is one of the most reliable biomarkers to indicate early biological effects ...
Anderson, Diana +2 more
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Environmental Pollution
The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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The paper provides the first assessment of the occurrence of hormetic dose responses using the Comet assay, a genotoxic assay. Using a priori evaluative criteria based on the Hormetic Database on peer-reviewed comet assay experimental findings, numerous examples of hormetic dose responses were obtained.
Edward J. Calabrese, Paul B. Selby
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Miniaturizing the comet assay with 3D vertical comets
Cytometry Part A, 2002AbstractBackgroundThe comet or single‐cell gel electrophoresis assay is a sensitive method for the detection of DNA damage. The main drawback of comet sampling is the low cell density necessary to prevent nucleus overlap after electrophoresis, which limits large‐scale high throughput screening. Another problem may be inconsistent comet focusing.
Philippe, Baert, Patrick, Van Oostveldt
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The Comet Assay in Clinical Practice
Acta Oncologica, 1999The comet assay is a single-cell gel electrophoresis technique that measures DNA damage in individual cells. Since radiation produces 3-4 times more DNA damage in well-oxygenated cells compared with hypoxic cells, this assay can quantify the fraction of radiation-resistant hypoxic cells found in many solid tumours.
P L, Olive +9 more
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2018
The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
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The comet assay is one of the techniques that has been extensively used to assess DNA damage in both the somatic cells and the spermatozoa over the past years. The results are robust and, as difference with other strategies to assess DNA damage, information about the presence of single-strand breaks (SSBs) or double-strand breaks (DSBs) can be obtained.
Elva I. Cortés-Gutiérrez +2 more
openaire +1 more source