Results 71 to 80 of about 2,077,783 (311)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Barcelona, a Society and its Law: 11th-13th Centuries
Catalan Historical Review, 2008 The legal system in Barcelona in the Lower Middle Ages — which we regard as a synthesis of rights (usages, customs, privileges, laws, and so on) and organization (municipality, universitat de prohoms, consulates, etc.)− had already attained maturity in ...
Tomàs de Montagut
doaj
Arqueología y Territorio Medieval, 2020 Aborder le thème des mobiliers céramiques glaçurés, qui constituent une part, somme toute, assez modeste des cargaisons étudiées des épaves islamiques de Provence, revêt toutefois une importance cruciale en terme de diffusion des techniques et savoir ...
Chaterine Richarté-Manfredi
doaj
Trabajos de Prehistoria, 1998 [es] Al sur de la desembocadura del río Segura, la excavación de La Rábita califal ha revelado la existencia, en el mismo lugar, de un establecimiento de época Orientalizante/Protoibérica e Ibérica Antigua. El hábitat, cuya historia empieza probablemente
Rafael Azuar +5 more
doaj +1 more source
The Challenge of Co-Religionist Commerce [PDF]
, 2014 This Article addresses the rise of co-religionist commerce in the United States—that is, the explosion of commercial dealings that take place between co-religionists who intend their transactions to achieve both commercial and religious objectives.
Fleetwood, Filippa +6 more
core +2 more sources
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Supply to the population in Kraljevo from 1911 to 1951 [PDF]
Baština, 2014 Kraljevo was one of the most destroyed town in Serbia during the Second World War. The town suffered a lot of causalities and significant destruction of infrastructure. All economic-related activities during the war were almost benumbed, and agricultural
Simijanović Jovan D.
doaj
Automation of Data Collection for Measuring The Quality of E-Commerce [PDF]
, 2013 The research aims to help managers in information gathering web log visitors based on traffic data, building automation data for determining the needs of e-Commerce extensibility, and design of information systems in the process of database interaction ...
Andi, Gunawan +2 more
core