Results 201 to 210 of about 134,408 (268)

Engineering Compact Base Editors by AlphaFold‐Guided Mutation Scan and Escherichia coli‐Based Tri‐Selection

open access: yesAdvanced Science, EarlyView.
A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son   +2 more
wiley   +1 more source

A Novel Curricular Approach to Lifestyle Medicine Education Through Shared Medical Appointment Simulations. [PDF]

open access: yesAm J Lifestyle Med
Bishop M   +8 more
europepmc   +1 more source

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