Results 91 to 100 of about 285,043 (352)

Studies on the Binding of Carboxypeptidase A and Several Competitive Inhibitors

Journal of Biological Chemistry, 1962
Several spectral changes have been reported to be related to changes in bonding or other factors in the environment of the side chains of aromatic amino acid residues (l-lo). It would then be expected that on addition of a competitive inhibitor to an enzyme solution, a change in the spectrum of the enzyme could be observed, if aromatic amino acid ...
H, FUJIOKA, K, IMAHORI
openaire   +2 more sources

Τhiazolidine-4-One Derivatives with Variable Modes of Inhibitory Action Against DPP4, a Drug Target with Multiple Activities and Established Role in Diabetes Mellitus Type II

Pharmaceuticals
Background/Objectives: DPP4 is an enzyme with multiple natural substrates and probable involvement in various mechanisms. It constitutes a drug target for the treatment of diabetes II, although, also related to other disorders.
Dionysia Amanatidou, Phaedra Eleftheriou, Anthi Petrou, Athina Geronikaki, Theodoros Lialiaris   +4 more
doaj   +1 more source

Non-specific binding of antibodies in immunohistochemistry: Fakes and facts

, 2011
Protocols for blocking non-specific antibody (Ab) binding in immunohistochemistry are based on rather contradictory and outdated reports. This prompted us to prove, whether non-specific Ab binding may really lead to unwanted background staining in ...
Vera Samoilova, Werner Boecker, Markus Tiemann, Igor Buchwalow   +3 more
core  

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Kinetics of Competitive Binding with Application to Thrombin Complexes

Analytical Biochemistry, 1997
The kinetics of competitive binding is treated analytically, allowing the rate constants to be determined accurately from simple experiments. The method is especially suited to situations where traditional approximations and numerical integration fail, e.g., when the dissociation constants are small or when the concentration of one receptor cannot be ...
W J, Wedemeyer, R W, Ashton, H A, Scheraga   +2 more
openaire   +2 more sources

Contribution of Active and Inactive States of the Human 5-HT4d Receptor to the Functional Activities of 5-HT4–Receptor Agonists

Journal of Pharmacological Sciences, 2008
In the present study, binding affinities of 5-hydroxytryptamine-4 (5-HT4) ligands for the human 5-HT4d receptor were determined using the agonist [3H]5-HT and the selective 5-HT4 antagonist [3H]GR113,808. We also compared the affinity differences between
Tadayoshi Mikami, Hiromi Sugimoto, Rie Naganeo, Takashi Ohmi, Toshiyuki Saito, Hiroyuki Eda   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Apparent dissociation constants for the binding of different sugars to MhPA14, using the competitive binding assay.

, 2019
Apparent dissociation constants for the binding of different sugars to MhPA14, using the competitive binding assay.
Peter L. Davies (121653), Shayan Assaie-Ardakany (7027148), Brigid Conroy (7027151), Shuaiqi Guo (121638), Tyler D. R. Vance (3365498)   +4 more
core   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Binding-Induced DNA Dissociation Assay for Small Molecules: Sensing Aflatoxin B1

, 2018
We describe a new fluorescence turn-on sensor for homogeneous detection of aflatoxin B1 (AFB1), a potent low molecular weight mycotoxin. A key innovation is the binding-induced intramolecular interaction involving the following two sets of probes: (1) a ...
Zhixin Wang (310470), Peiwu Li (510514), Zhaowei Zhang, Lin Xu, Hongquan Zhang (61781), Hongquan Zhang, Hanyong Peng (2389192), Zhaowei Zhang (510517), Qi Zhang, Peiwu Li, Hanyong Peng, Zhixin Wang, Xiaowen Yan, Lin Xu (94491), X. Chris Le (17762), X. Chris Le, Xiaowen Yan (1820854), Qi Zhang (28502)   +17 more
core   +1 more source