Results 301 to 310 of about 2,034,857 (344)

Analysis of complement Factor B activation by PAGE-immunoblotting

Journal of Immunological Methods, 1989
Initiation of complement activation via the alternative pathway results in cleavage of Factor B into two fragments Ba and Bb. Employing PAGE and immunoblotting analysis we have demonstrated Ba and Bb activation fragments following in vitro and in vivo activation in a range of biological fluids including cerebrospinal fluid and synovial fluid ...
M. Doran, M. Doran, D.J. Reen
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Hormonal Regulation of Complement Factor B in Human Endometrium

American Journal of Reproductive Immunology, 1993
PROBLEM: Recent investigations have demonstrated the presence of complement components in human endometrium in a cycle‐specific manner. Luteal phase endometrium has been shown to synthesize complement C3 de novo, whereas proliferative endometrium produces little or no C3. Likewise factor B. which is critical to the activation of the alternative pathway
Wendy W. Brockman, Lisa A. Hasty, C. Richard Lyttle, John D. Lambris   +3 more
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Complement factor H and factor B expression in RPE cells

Acta Ophthalmologica, 2008
Abstract Purpose Age‐related macular degeneration (AMD) is the leading cause of untreatable blindness in the developed world. The pathogenesis of AMD is not fully understood. Recent evidence suggests that local inflammation in particular complement activation plays an important role. We aim to understand how complement activation is regulated at retina/
Heping Xu, Mei Chen, J.V. Forrester
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Activation of the alternative pathway of complement by antiserum to factor B.

The Journal of laboratory and clinical medicine, 1979
Monospecific rabbit and goat antisera to human complement proteins and human immunoglobulins were tested for their ability to activate the alternative complement pathway. This activation was detected by two methods where classical pathway activation was blocked with EGTA and alternative pathway activation was promoted with added magnesium ions.
Eng M. Tan, Merlin R. Wilson, Carlos M. Arroyave   +2 more
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Application of champedak mannose‐binding lectin in the glycoproteomic profiling of serum samples unmasks reduced expression of alpha‐2 macroglobulin and complement factor B in patients with nasopharyngeal carcinoma

Electrophoresis, 2010
The use of lectin affinity chromatography prior to 2‐DE separation forms an alternative method to unmask the expression of targeted glycoproteins of lower abundance in serum samples.
Ramarao Seriramalu, Wei Wei Pang, J. J. Jayapalan, Emida Mohamed, P. S. Abdul-Rahman, A. Bustam, A. Khoo, O. Hashim   +7 more
semanticscholar   +1 more source

Studies on esterolytic activity of alternative complement component factor B

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983
Abstract Alternative complement component factor B was purified from human plasma and its esterolytic activity on various α-naphthylester derivatives was examined. Of these substrates, LeuAlaArg-naphthylester was the most susceptible. The K m value of factor B for this substrate was about 5·10 −4 M.
Michio Niinobe, Setsuro Fujii, Nobuhiko Ikari, Yuji Hitomi   +3 more
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Structure and activation of complement components C2 and Factor B

Philosophical Transactions of the Royal Society of London. B, Biological Sciences, 1984
The activation of complement is initiated by two independent pathways. Each leads to the formation of a complex protease, C3 convertase, with equivalent specificity and function but different composition. The convertase derived from the classical pathway is composed of complement components C4 and C2 while that from the alternative pathway consists of ...
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An assay for complement factor B in species at different levels of evolution

Developmental & Comparative Immunology, 1991
The alternative complement pathway is regarded as the evolutionary ancestral complement system. It is therefore of interest to analyze and characterize factor B of the alternative complement pathway in animals at different levels of evolution. We describe a functional assay which is based on the interaction of factor B from various animal species with ...
Claus Koch, Lisbeth Bjerring Jensen
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A New Complement Factor B Variant (BF S075) in Japanese

Human Heredity, 1990
A new slow-moving variant of the complement factor B, named BF S075, was found in a Japanese patient with cerebral thrombosis and urticaria. The variant was inherited in a codominant manner. The protein concentration and functional hemolytic activity of the complement factor B in the patient's serum were within normal limits.
Takaaki Shinomiya, Ikuro Maruyama, Satoshi Takenaga, Hiroaki Nishimukai, Hajime Kitamura, Kenji Mizutani   +5 more
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Amyloid‐β up‐regulates complement factor B in retinal pigment epithelial cells through cytokines released from recruited macrophages/microglia: Another mechanism of complement activation in age‐related macular degeneration

Journal of Cellular Physiology, 2009
One of the earliest signs of age‐related macular degeneration (AMD) is the formation of drusen which are extracellular deposits beneath the retinal pigmented epithelium (RPE).
Jiying Wang, K. Ohno-Matsui, Takeshi Yoshida, N. Shimada, S. Ichinose, Tetsuji Sato, M. Mochizuki, I. Morita   +7 more
semanticscholar   +1 more source