A diverse semisynthetic humanized scFv phage display library for anti-CXCL16 antibodies. [PDF]
J Biol ChemLi Z, Chen Q, Wang S, Chen J, Chen S.
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Determination of framework regions (FRs) and complementarity determining regions (CDRs) in an antibody is essential for understanding the underlying biology as well as antibody engineering and optimization. However, there are no computational algorithms available to delimit an antibody sequence or a library of sequences into FRs and CDRs in a coherent ...
Shanrong Zhao, Jin Lu
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Cluster and information entropy patterns in immunoglobulin complementarity determining regions
BioSystems, 2004Previous studies of antibody binding domains have established many crucial features that include important structural positions, canonical formations, and the geometric correlations with the binding site nature and topography. In this work, position-specific frequency and hierarchical clustering analysis are used to explore the statistical pattern of ...
Stephanie, Culler +3 more
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Replacing the complementarity-determining regions in a human antibody with those from a mouse
Nature, 1986The variable domains of an antibody consist of a beta-sheet framework with hypervariable regions (or complementarity-determining regions--CDRs) which fashion the antigen-binding site. Here we attempted to determine whether the antigen-binding site could be transplanted from one framework to another by grafting the CDRs. We substituted the CDRs from the
Peter T Jones +2 more
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Heavy-Chain Complementarity-Determining Regions Determine Conformation Selectivity of Anti-Aβ Antibodies [PDF]
Neurodegenerative Diseases, 2010 <i>Background/Aims:</i> Amyloid-β (Aβ) protofibrils are neurotoxic soluble intermediates in the Aβ aggregation process eventually forming senile plaques in Alzheimer’s disease. This Aβ species is a potential biomarker for Alzheimer’s disease and also a promising target for immunotherapy. In this study, we investigated the characteristics of
Dag, Sehlin +7 more
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Downsizing antibodies: Towards complementarity-determining region (CDR)-based peptide mimetics [PDF]
Bioorganic Chemistry, 2022 Monoclonal antibodies emerged as an important therapeutic drug class with remarkable specificity and binding affinity. Nonetheless, these heterotetrameric immunoglobulin proteins come with high manufacturing and therapeutic costs which can take ...
Kevin Van Holsbeeck +2 more
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Exploiting sequence space: shuffling in vivo formed complementarity determining regions into a master framework [PDF]
Gene, 1998 A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region.
Mats Ohlin, C A Borrebaeck
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The His-probe method: Effects of histidine residues introduced into the complementarity-determining regions of antibodies on antigen-antibody interactions at different pH values [PDF]
FEBS Letters, 1992 We examined the effects or histidine residues that were artificially introduced into complementarity-determining regions of antibodies on antigen-antibody Interactions at different pH values.
Wataru Itô, Nobuo Sakato, Yoji Arata
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In the past, specificity and affinity were the priority for synthetic antibody library. However, therapeutic antibodies need good stability for medical use. Through carefully adjust the chemical diversity in CDRs, one hopes to design a synthetic antibody library with good developability. Here we thoroughly analyzed 296 nanobody sequences and structures,
Zhenwei, Zhong +6 more
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