Results 71 to 80 of about 25,476 (265)

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Fab Fragment of V_HH-Based Antibody Netakimab: Crystal Structure and Modeling Interaction with Cytokine IL-17A

Crystals, 2019
Interleukin 17A (IL-17A) is a proinflammatory cytokine produced by Th17 cells. Antibody BCD-085 (netakimab) against human IL-17A is one of the new inhibitors of this cytokine.
Olga Kostareva, Ilya Kolyadenko, Andrey Ulitin, Victoria Ekimova, Stanislav Evdokimov, Maria Garber, Svetlana Tishchenko, Azat Gabdulkhakov   +7 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

In Silico Prediction Analysis of Idiotope-Driven T–B Cell Collaboration in Multiple Sclerosis

Frontiers in Immunology, 2017
Memory B cells acting as antigen-presenting cells are believed to be important in multiple sclerosis (MS), but the antigen they present remains unknown.
Rune A. Høglund, Rune A. Høglund, Andreas Lossius, Andreas Lossius, Jorunn N. Johansen, Jane Homan, Jūratė Šaltytė Benth, Jūratė Šaltytė Benth, Harlan Robins, Bjarne Bogen, Bjarne Bogen, Bjarne Bogen, Robert D. Bremel, Trygve Holmøy, Trygve Holmøy   +14 more
doaj   +1 more source

Cell geometry and membrane protein crowding constrain Escherichia coli growth rate, overflow metabolism, respiration, and maintenance energy

FEBS Letters, EarlyView.
The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson, Tomáš Gedeon, Mauricio Garcia Benitez, Campbell Putnam, William R. Harcombe, Radhakrishnan Mahadevan, Ashley E. Beck   +6 more
wiley   +1 more source

A competitive binding-mass spectrometry strategy for high-throughput evaluation of potential critical quality attributes of therapeutic monoclonal antibodies

mAbs, 2022
Therapeutic monoclonal antibodies (mAbs) have a propensity to host a large number of chemical and enzymatical modifications that need to be properly assessed for their potential impact on target binding.
Zhengqi Zhang, Yuetian Yan, Shunhai Wang, Ning Li   +3 more
doaj   +1 more source

Mixed‐class J‐domain protein scaffolds promote expanded aggregate handling and multivalent Hsp70 engagement during functional disaggregase assembly

FEBS Letters, EarlyView.
Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley   +1 more source

Impact of the high tyrosin fraction in complementarity determining regions: measured and predicted effects of radioiodination on IgG immunoreactivity [PDF]

, 1995
Although iodine-131 is the most widely used radionuclide for radioimmunotherapy, direct radiolabeling methods yield decreased immunoreactivity of the antibody as a function of increased iodine incorporation.
CURCIO M. J., BOCCHIA M., NIKULA K. T., SCHEINBERG D. A., MA Y., SGOUROS G., FIN R. D.   +6 more
core   +1 more source

Image_1_Complementarity determining regions in SARS-CoV-2 hybrid immunity.jpg [PDF]

, 2023
Pre-vaccination SARS-CoV-2 infection can boost protection elicited by COVID-19 vaccination and post-vaccination breakthrough SARS-CoV-2 infection can boost existing immunity conferred by COVID-19 vaccination.
Xavier Bossuyt (564860), Lise Boon (3901150), Glynis Frans (13520773), Kusay Arat (14655164), Jonas De Leeuw (14655152), Lina Ancheva (14655158), Nick Geukens (638409), Rita Derua (2007325), Jan Van Elslande (12877076), Bas Calcoen (14655155), Wim Maes (3826516), Nico Callewaert (317511), Pieter Vermeersch (4747938), Sebastien Christian Carpentier (1273161), Tom Dehaemers (14655149), Maaike Cockx (14655161), Doreen Dillaerts (14655146)   +16 more
core   +1 more source

Bovine antibodies with ultra long H3 Complementarity Determining Regions [PDF]

Acta Crystallographica Section A Foundations and Advances, 2014
About 10% of the bovine antibody repertoire exhibit extremely long H3 complementarity determining regions (CDRs). These H3 CDRs are usually described as `loops' in the more familiar mouse and human antibody Fab structures, but the ultra long bovine H3 CDRs are actually small, cysteine-rich protein domains that vary in size from 44 to 64 amino acids. We
Robyn Stanfield, Vaughn Smider, Ian Wilson   +2 more
openaire   +1 more source