Results 61 to 70 of about 25,476 (265)

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Amino acid sequences of the V_H regions of #37 and #55. [PDF]

, 2013
Residues identical to #37 sequences are indicated by a dash. Dots indicate gaps compared with the sequence of #37. MAb, monoclonal antibody; FR, framework regions; CDR, complementarity-determining regions.
Robert H. Purcell (284615), Masamichi Oshima (284610), Yohko K. Shimizu (284609), Hiroshi Yoshikura (284617), Hak Hotta (127864), Minako Hijikata (159457), Kazufumi Shimizu (284611), Harvey J. Alter (284613)   +7 more
core   +1 more source

Structural Diversity of Ultralong CDRH3s in Seven Bovine Antibody Heavy Chains

Frontiers in Immunology, 2019
Antigen recognition by mammalian antibodies represents the most diverse setting for protein-protein interactions, because antibody variable regions contain exceptionally diverse variable gene repertoires of DNA sequences containing combinatorial, non ...
Jinhui Dong, Jessica A. Finn, Peter A. Larsen, Timothy P. L. Smith, James E. Crowe, James E. Crowe, James E. Crowe   +6 more
doaj   +1 more source

Development of antibody-dependent cell cytotoxicity function in HIV-1 antibodies

eLife, 2021
A prerequisite for the design of an HIV vaccine that elicits protective antibodies is understanding the developmental pathways that result in desirable antibody features. The development of antibodies that mediate antibody-dependent cellular cytotoxicity
Laura E Doepker, Sonja Danon, Elias Harkins, Duncan K Ralph, Zak Yaffe, Meghan E Garrett, Amrit Dhar, Cassia Wagner, Megan M Stumpf, Dana Arenz, James A Williams, Walter Jaoko, Kishor Mandaliya, Kelly K Lee, Frederick A Matsen IV, Julie M Overbaugh   +15 more
doaj   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source

Molecular Insights of Nickel Binding to Therapeutic Antibodies as a Possible New Antibody Superantigen

Frontiers in Immunology, 2021
The binding of nickel by immune proteins can manifest as Type IV contact dermatitis (Ni-specific T cells mediated) and less frequently as Type I hypersensitivity with both mechanisms remaining unknown to date.
Chinh Tran-To Su, Wai-Heng Lua, Jun-Jie Poh, Wei-Li Ling, Joshua Yi Yeo, Samuel Ken-En Gan, Samuel Ken-En Gan, Samuel Ken-En Gan   +7 more
doaj   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Do antibody CDR loops change conformation upon binding?

mAbs
Antibodies have increasingly been developed as drugs with over 100 now licensed in the US or EU. During development, it is often necessary to increase or reduce the affinity of an antibody and rational attempts to do so rely on having a structure of the ...
Chu’nan Liu, Lilian M. Denzler, Oliver E.C. Hood, Andrew C.R. Martin   +3 more
doaj   +1 more source

In Silico and Structural Analyses Demonstrate That Intrinsic Protein Motions Guide T Cell Receptor Complementarity Determining Region Loop Flexibility

Frontiers in Immunology, 2018
T-cell immunity is controlled by T cell receptor (TCR) binding to peptide major histocompatibility complexes (pMHCs). The nature of the interaction between these two proteins has been the subject of many investigations because of its central role in ...
Christopher J. Holland, Christopher J. Holland, Bruce J. MacLachlan, Valentina Bianchi, Valentina Bianchi, Sophie J. Hesketh, Sophie J. Hesketh, Richard Morgan, Owen Vickery, Anna M. Bulek, Anna Fuller, Andrew Godkin, Andrew K. Sewell, Pierre J. Rizkallah, Stephen Wells, David K. Cole, David K. Cole   +16 more
doaj   +1 more source

Length of the antibody heavy chain complementarity determining region 3 as a specificity‐determining factor

Journal of Molecular Recognition, 2004
AbstractThe antigen binding site of an antibody is made up of residues residing in six hypervariable loops of the heavy and light chains. In most cases several or all of these loops are required for the establishment of the antigen‐binding surface. Five of these loops display a limited diversity in length and sequence while the third complementarity ...
Barrios del Pino, Yvelise, Jirholtz, Pernilla, Ohlin, Mats   +2 more
openaire   +3 more sources