Results 31 to 40 of about 2,891,675 (191)

Rapid, highly sensitive and highly specific gene detection by combining enzymatic amplification and DNA chip detection simultaneously

Sensing and Bio-Sensing Research, 2016
We have developed a novel gene detection method based on the loop-mediated isothermal amplification (LAMP) reaction and the DNA dissociation reaction on the same DNA chip surface to achieve a lower detection limit, broader dynamic range and faster ...
Koji Hashimoto, Naoko Nakamura, Keiko Ito   +2 more
doaj  

Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

Sensors, 2018
The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming.
Yesong Gu, Po-Yuan Tseng, Xiang Bi, Jason H. C. Yang   +3 more
doaj   +1 more source

Structural Landscape of the Transition from an ssDNA Dumbbell Plus Its Complementary Hairpin to a dsDNA Microcircle Via a Kissing Loop Intermediate

Molecules, 2021
The experimental construction of a double-stranded DNA microcircle of only 42 base pairs entailed a great deal of ingenuity and hard work. However, figuring out the three-dimensional structures of intermediates and the final product can be particularly ...
Alberto Mills, Federico Gago
doaj   +1 more source

Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense

Biosensors, 2013
An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen.
Noremylia Mohd Bakhori, Nor Azah Yusof, Abdul Halim Abdullah, Mohd Zobir Hussein   +3 more
doaj   +1 more source

Interdigitated Electrode Biosensor Based on Plasma-Deposited TiO₂ Nanoparticles for Detecting DNA

Biosensors, 2021
Bioelectrodes mediated by metal oxide nanoparticles have facilitated the development of new sensors in medical diagnosis. High-purity TiO2 nanoparticles (NPs) were synthesized through thermal plasma and deposited directly on an interdigitated electrode ...
Jhongryul Yoo, Hongin Jeong, Seo Kyung Park, Sungho Park, Je Seung Lee   +4 more
doaj   +1 more source

Bioelectronic DNA detection of human papillomaviruses using eSensor™: a model system for detection of multiple pathogens

BMC Infectious Diseases, 2003
Background We used human papillomaviruses (HPV) as a model system to evaluate the utility of a nucleic acid, hybridization-based bioelectronic DNA detection platform (eSensor™) in identifying multiple pathogens.
Miller Donna L, Chan Vivian, Unger Elizabeth R, Farkas Daniel H, Vernon Suzanne D, Chen Yin-Peng, Blackburn Gary F, Reeves William C   +7 more
doaj   +1 more source

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

International Journal of Analytical Chemistry, 2020
Trans-Zeatin is the major active phytohormone in immature corn kernels. Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-zeatin was constructed.
Ping Sun, Xiwei Zhang, Xianxiang Wang
doaj   +1 more source

RecA filament sliding on DNA facilitates homology search

eLife, 2012
During homologous recombination, RecA forms a helical filament on a single stranded (ss) DNA that searches for a homologous double stranded (ds) DNA and catalyzes the exchange of complementary base pairs to form a new heteroduplex.
Kaushik Ragunathan, Cheng Liu, Taekjip Ha   +2 more
doaj   +1 more source

Hierarchical coassembly of DNA–triptycene hybrid molecular building blocks and zinc protoporphyrin IX

Beilstein Journal of Nanotechnology, 2016
Herein, we describe the successful construction of composite DNA nanostructures by the self-assembly of complementary symmetrical 2,6,14-triptycenetripropiolic acid (TPA)–DNA building blocks and zinc protoporphyrin IX (Zn PpIX).
Rina Kumari, Sumit Singh, Mohan Monisha, Sourav Bhowmick, Anindya Roy, Neeladri Das, Prolay Das   +6 more
doaj   +1 more source

Identification of a criminal by DNA typing in a rape case in Rio de Janeiro, Brazil

São Paulo Medical Journal, 2002
CONTEXT: Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains.
Andréa Carla de Souza Góes, Dayse Aparecida da Silva, Cristiane Santana Domingues, João Marreiro Sobrinho, Elizeu Fagundes de Carvalho   +4 more
doaj   +1 more source