Results 71 to 80 of about 7,947,791 (315)

Spliceator: multi-species splice site prediction using convolutional neural networks

BMC Bioinformatics, 2021
Background Ab initio prediction of splice sites is an essential step in eukaryotic genome annotation. Recent predictors have exploited Deep Learning algorithms and reliable gene structures from model organisms.
Nicolas Scalzitti, Arnaud Kress, Romain Orhand, Thomas Weber, Luc Moulinier, Anne Jeannin-Girardon, Pierre Collet, Olivier Poch, Julie D. Thompson   +8 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Data-Driven Quality Assurance: Defect Prediction to Reduce Carbon Footprint in Manufacturing Logistics [PDF]

E3S Web of Conferences
In modern manufacturing environments, minimizing carbon emissions while maintaining product quality has become a strategic imperative. One critical yet often overlooked contributor to elevated CO₂ emissions is the occurrence of unexpected quality defects,
Douiri Rokaya, Taraa Chaymae, Elmoussami Haj, Ezzine Latifa   +3 more
doaj   +1 more source

Transparency in Complex Computational Systems [PDF]

, 2019
Scientists depend on complex computational systems that are often ineliminably opaque, to the detriment of our ability to give scientific explanations and detect artifacts. Some philosophers have s..
Creel, Kathleen A.
core   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Controlling protein activity by dynamic recruitment on a supramolecular polymer platform

Nature Communications, 2018
DNA-origami allows the precise recruitment of DNA-protein conjugates but lacks the dynamics found in natural protein assemblies. Here the authors present a synthetic polymer platform that combines the dynamics of supramolecular polymers with the ...
Sjors P. W. Wijnands, Wouter Engelen, René P. M. Lafleur, E. W. Meijer, Maarten Merkx   +4 more
doaj   +1 more source

Community characterization of heterogeneous complex systems

, 2010
We introduce an analytical statistical method to characterize the communities detected in heterogeneous complex systems. By posing a suitable null hypothesis, our method makes use of the hypergeometric distribution to assess the probability that a given ...
, , Fabrizio Lillo, Feller W, Jan Varho, Jyrki Piilo, McCallum A, Michele Tumminello, Miller R G, Onnela J P, Rosario N Mantegna, Salvatore Miccichè, Tumminello M Micciché S Lillo F Piilo J Mantegna R N   +12 more
core   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

The shocklet transform: a decomposition method for the identification of local, mechanism-driven dynamics in sociotechnical time series

EPJ Data Science, 2020
We introduce a qualitative, shape-based, timescale-independent time-domain transform used to extract local dynamics from sociotechnical time series—termed the Discrete Shocklet Transform (DST)—and an associated similarity search routine, the Shocklet ...
David Rushing Dewhurst, Thayer Alshaabi, Dilan Kiley, Michael V. Arnold, Joshua R. Minot, Christopher M. Danforth, Peter Sheridan Dodds   +6 more
doaj   +1 more source

What Makes Complex Systems Complex? [PDF]

, 2018
This paper explores some of the factors that make complex systems complex. We first examine the history of complex systems. It was Aristotle’s insight that how elements are joined together helps determine the properties of the resulting whole. We find (a)
Abbott, Russ
core