Results 131 to 140 of about 20,421,622 (264)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Composite Materials Engineering Graduating Class Photograph: 2011

open access: yes, 2011
This is a class photograph of the Composite Materials Engineering Graduating Class of 2011 at Winona State University. The students in the photograph are: Eric A.
Composite Materials Engineering Department-Winona State University
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Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty   +13 more
wiley   +1 more source

Shear strength and moment-shear interaction of steel-concrete composite beams:Experiments, numerical analyses, and design models

open access: yes, 2012
Current structural codes do not address the shear strength and the moment-shear interaction in composite beams explicitly. The shear is commonly assumed to be resisted by the steel beam alone neglecting the contributions of the slab and the composite ...
Uy, Brian (R9735)   +3 more
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Composite Materials Engineering Graduating Class Photograph: 2020

open access: yes, 2020
This is a class photograph of the Composite Materials Engineering Graduating Class of 2020 at Winona State University. The students in the photograph are: Makaylan Benz, Braxton Bliese, Kevin Eischens, Alexander Evans, Oussama Ezzaher, Nicholas Henderson,
Composite Materials Engineering Department-Winona State University
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Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

open access: yesFEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima   +7 more
wiley   +1 more source

Computer construction of quasi-twisted two-weight codes

open access: yes, 2009
A code is said two-wight if the non-zero codewords have only two different weights w1 and w2. In this paper, it is generalized that a consta-cyclic code of composite length can be put in the quasi-twisted (QT) form.
Chen, Eric Zhi,
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Heterotropic regulation and negative homotropic cooperativity

open access: yesFEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea   +5 more
wiley   +1 more source

Steel-concrete composite beams behaviour under combined hogging moment and axial tension

open access: yes, 2011
Composite steel-concrete beams are used in many types of structures, including steel framed buildings, bridges, stadiums and car parks. Composite beams are being increasingly used in situations where combinations of bending, shear and axial force may be ...
Uy, Brian (R9735)   +3 more
core  

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