Results 41 to 50 of about 75,962 (250)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

MPAS‐Ocean Simulation Quality for Variable‐Resolution North American Coastal Meshes

Journal of Advances in Modeling Earth Systems, 2020
Climate model components utilizing unstructured meshes enable variable resolution, regionally enhanced simulations within global domains. Here we investigate the relationship between mesh quality and simulation statistics using the JIGSAW unstructured ...
Kristin E. Hoch, Mark R. Petersen, Steven R. Brus, Darren Engwirda, Andrew F. Roberts, Kevin L. Rosa, Phillip J. Wolfram   +6 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Effect of secondary swirl in supersonic gas and plasma flows in the self-vacuuming vortex tube

MATEC Web of Conferences, 2018
This article presents the results of simulation for a special type of vortex tubes – self-vacuuming vortex tube (SVT), for which extreme values of temperature separation and pressure drop are realized.
Volov Vyacheslav, Lyaskin Anton
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Comparative numerical studies of the mechanical efficiency of sandwich-panels with different non-/auxetic prismatic and lattice cores subjected to ballistic loading

Forces in Mechanics
Civil as well as military facilities, vehicles and applications must increasingly meet higher safety standards to ensure the highest possible protection against extraordinary stresses such as ballistic and/or air-blast loading.
Marcel Walkowiak, Denis Anders, Ulf Reinicke   +2 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Influence of Viscosity on the Shape of an Air Taylor Bubble in a Stagnant Liquid Under Turbulent Condition in Falling Film

International Journal of Applied Mechanics and Engineering, 2019
This work aims to find the influence of the liquid viscosity on the shape of an air Taylor bubble, rising up in a pipe column which contains the liquid under conditions that the liquid is stagnant and the Froude number is approximately equal to 0.35 ...
B. Lertnuwat
doaj   +1 more source