Results 41 to 50 of about 321,627 (335)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Conserved recombination patterns across hepatitis B genotypes: a retrospective study

Virology Journal
Hepatitis B virus (HBV) infection is a major public health concern, as chronic HBV infection can lead to liver cirrhosis and increase a person’s risk of developing hepatocellular carcinoma (HCC). HBV has been classified into ten genotypes (A to J). Here,
Derek Tshiabuila, James E. San, Eduan Wilkinson, Graeme Dor, Houriiyah Tegally, Tongai G. Maponga, Marion Delphin, Philippa C. Matthews, Darren P. Martin, Cheryl Baxter, Tulio de Oliveira   +10 more
doaj   +1 more source

ЕЛЕМЕНТИ ПІДГОТОВКИ МАЙБУТНІХ УЧИТЕЛІВ ІНФОРМАТИКИ ДО ЗАСТОСУВАННЯ ТЕХНОЛОГІЇ ФОРМУВАННЯ COMPUTATIONAL THINKING

Фізико-математична освіта, 2021
Формування Computational Thinking у здобувачів освіти є одним з основних завдань сучасного педагога. Застосування технології формування Computational Thinking у навчанні майбутніх учителів інформатики дозволить на практиці опанувати дану технологію та в ...
М. Медведєва, О. Жмурко, І. Криворучко, М. Ковтанюк   +3 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Dolutegravir rollout for treatment of HIV with a focus on advanced disease and tuberculosis coinfection: findings from rural KwaZulu-Natal, South Africa (2019–2023)

AIDS Research and Therapy
Background While Dolutegravir (DTG) containing antiretroviral therapy (ART) has become the preferred regimen for people living with HIV (PLHIV), the pace and equity of its adoption, especially among subgroups with tuberculosis (TB) symptoms and advanced ...
Reuben Christopher Moyo, Larisse Bolton, Elphas Luchemo Okango, Margot Otto, Nthoesele Letoao, Peter Suwirakwenda Nyasulu, Frank Tanser   +6 more
doaj   +1 more source

Peer network approaches for improving HIV testing, prevention and care utilisation among men in sub-Saharan Africa: a scoping review

BMJ Open
Introduction Limited male engagement in HIV prevention and care is a global challenge more pronounced in sub-Saharan Africa (SSA) contributing to ongoing transmission.
Guy Harling, Frank Tanser, Paul Mee, Maxime Inghels, Khai Hoan Tram, Wilfred Ouma Otambo, Margot Otto, Ntombifuthi Blose   +7 more
doaj   +1 more source

Computational Thinking und Kontextorientierung.

Medienimpulse, 2020
Je nach Perspektive wird in der digitalen Bildung über digitale Medien, über Medienbildung, über informationstechnische Grundbildung, über Informatik, Problemlösefähigkeiten, Schlüsselkompetenzen und technische Allgemeinbildung gesprochen.
Michael Steiner, Klaus Himpsl-Gutermann
doaj   +1 more source

Nonexistence of Chaotic Solutions of Nonlinear Differential Equations

, 2011
We discuss some important issues arising from computational efforts in dynamical systems and fluid dynamics. Various individuals have misunderstood these issues since the onset of these problem areas; indeed, they have been routinely misinterpreted, and ...
Yao, Lun-Shin
core   +1 more source

Searching for Early Developmental Activities Leading to Computational Thinking Skills [PDF]

, 2017
Drawing on the long debate about whether computer science (CS) and computational thinking skills are innate or learnable, this working group is based on the following hypothesis: The apparent innate ability of some CS learners who succeed in CS courses ...
Alshaigy, Bedour, Cole, Elizabeth, Cutts, Quintin, Donaldson, Peter, Gutica, Mirela, Hellas, Arto, Larraza-Mendiluze, Edurne, McCartney, Robert, Patitsas, Elizabeth, Riedesel, Charles   +9 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source