Results 131 to 140 of about 10,839,512 (369)

Purification tags markedly affect self‐aggregation of CPEB3

open access: yesFEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito   +6 more
wiley   +1 more source

What is Computer Science? The two crises of computer science.

open access: yes, 2019
What is Computer Science? The two crises ofcomputer science. A second, habilitation thesis.
openaire   +2 more sources

Circulating histones as clinical biomarkers in critically ill conditions

open access: yesFEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez   +17 more
wiley   +1 more source

A log analysis based intrusion detection system for the creation of a specification based intrusion prevention system [PDF]

open access: yes, 2003
We propose a novel Intrusion Prevention System (IPS) which would base its knowledge and operation on a higher level of abstraction than the processing of the contents of the network packets audit data themselves which is the source of data on which most ...
1st Computer Science Annual Workshop (CSAW’03)   +1 more
core  

Flipped Computer Science Classes [PDF]

open access: yesAnnals of computer science and information systems, 2014
R. Robert Gajewski, Marcin Jaczewski
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

open access: yesFEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao   +7 more
wiley   +1 more source

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