Results 101 to 110 of about 540,654 (303)

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Expression of Procollagen A1 Type I Induced by Two Different Dentine Bonding Systems in Human Pulp Fibroblasts

European Journal of Inflammation, 2013
This study aimed to evaluate the effects of two different dentine bonding systems (DBSs) on primary cultures of human pulp fibroblasts (HPFs). Cell viability and procollagen α1 type I expression were investigated.
G. Orsini, M. Mattioli-Belmonte, M. Orciani, A. Mazzoni, G. Teti, M. Falconi, A. Putignano, L. Breschi   +7 more
doaj   +1 more source

Selective wear of cuttings elements of working organs of tillage machines with realization of self-sharpening effect [PDF]

, 2013
The clause of strengthening of cuttings elements is conditioning and realization of such selforganization process as a self-sharpening. Sharpening of horizontal and vertical cuttings elements of working organs of tillage machines (CE WOTM) is considered ...
Aulin, V., Zamota, T., Аулін, В. В.   +2 more
core  

Microenvironmental influence on microtumour infiltration patterns: 3D-mathematical modelling supported by: In vitro studies [PDF]

, 2018
Mathematical modelling approaches have become increasingly abundant in cancer research. Tumour infiltration extent and its spatial organization depend both on the tumour type and stage and on the bio-physicochemical characteristics of the ...
Calvo, Juan Carlos, Guerra, Liliana Noemi, Luján, Emmanuel, Marshall, Guillermo Ricardo, Rosito, María Sol, Soba, Alejandro, Soto, Daniela, Suárez, Cecilia Ana   +7 more
core   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Evaluation of the effect of mesenchymal stromal cells from different sources on human chondrocyte proliferation

Вестник трансплантологии и искусственных органов
Objective: to study the effect of a conditioned medium of mesenchymal stromal cells (MSCs) from different sources on human chondrocyte proliferation.Materials and methods.
A. D. Belova, Yu. B. Basok, A. M. Grigoriev, L. A. Kirsanova, E. G. Kuznetsova, I. V. Vakhrushev, A. V. Kovalev, K. N. Yarygin, V. I. Sevastianov   +8 more
doaj   +1 more source

Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria [PDF]

, 2016
Here, we provide the first evidence of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-vis supported the biosynthesis. TEM analysis evidenced that
Almeida-Paes, Rodrigo, de Souza, Wanderley, Eugenio, Mateus, Farina, Marcos, Frasés, Susana, Lemgruber, Leandro, Lima, Luís Maurício T.R., Müller, Nathalia, Sant'Anna, Celso   +8 more
core   +1 more source

Diversity and complexity in neural organoids

FEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

POTENSI TRANSDIFERENSIASI SEL FIBROBLAS MENJADI SEL SARAF SECARA IN VITRO (Transdifferentiation Potency of Fibroblast Cell to Neuron Cell in Vitro)

Jurnal Kedokteran Hewan, 2016
The aim of this study was to examine the potency of fibroblast cells transdifferentiated to neuron cells in vitro. Newborn rat neuron conditioned medium (NBRN CM) was collected from neuron cells cultured with mDMEM without serum for 48 hours.
Ekayanti M. Kaiin, Ita Djuwita
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source