Results 221 to 230 of about 2,348,563 (303)

Conditioned medium from human adipose-derived mesenchymal stromal cells can modulate cell migration and morphology of keratinocytes in vitro. [PDF]

Hum Cell
Rodrigues C, Paim TC, Zanatelli C, Prignon EVS, Azevedo JG, Naasani LIS, Wink MR.   +6 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Preclinical studies of conditioned medium of hypoxic-preconditioned mesenchymal stem cell/hypMSC-CM for ischemic stroke: A systematic review. [PDF]

Open Vet J
Rinendyaputri R, Nikmah UA, Panjaitan NSD, Intan PR, Situmorang JH, Idrus HH, Rilianawati R, Ayuningtyas W, Noverina R, Huda F, Faried A.   +10 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Comparative regenerative mechanisms of adipose-derived mesenchymal stem cell- and conditioned medium-loaded three-dimensional bioprinted hydrogels in chronic diabetic wounds. [PDF]

Regen Biomater
Wang L, Li X, Zhang X, Chu Q, Ye L, Jin T, Wang J, Sun Y, Wu S, Li T.   +9 more
europepmc   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Driving Naive State Induction Using Human Wharton Jelly-Mesenchymal Stem Cell-Derived Conditioned Medium in Rhesus Monkey Embryonic Stem Cells. [PDF]

Cells
Anwised P, Moorawong R, Samruan W, Srisutush J, Somredngan S, Aksoy I, Savatier P, Parnpai R.   +7 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

An effective approach to cartilage regeneration using antler stem cell-conditioned medium. [PDF]

Sci Rep
Zhou J, Xing B, Guo Q, Zhao J, Li X, Wang D, Yue Z, Lv J, Jiang Y, Sun H.   +9 more
europepmc   +1 more source