Results 21 to 30 of about 739,261 (262)

Evaluating performance in three-dimensional fluorescence microscopy [PDF]

open access: yes, 2007
In biological fluorescence microscopy, image contrast is often degraded by a high background arising from out of focus regions of the specimen. This background can be greatly reduced or eliminated by several modes of thick specimen microscopy, including ...
JOHN M. MURRAY   +7 more
core   +1 more source

An update: improvements in imaging perfluorocarbon-mounted plant leaves with implications for studies of plant pathology, physiology, development and cell biology.

open access: yesFrontiers in Plant Science, 2014
Plant leaves are optically complex, which makes them difficult to image by light microscopy. Careful sample preparation is therefore required to enable researchers to maximise the information gained from advances in fluorescent protein labelling, cell ...
George R Littlejohn   +9 more
doaj   +1 more source

Intraoperative fluorescence imaging for personalized brain tumor resection: Current state and future directions

open access: yesFrontiers in Surgery, 2016
Introduction: Fluorescence-guided surgery is one of the rapidly emerging methods of surgical theranostics. In this review, we summarize current fluorescence techniques used in neurosurgical practice for brain tumor patients, as well as future ...
Evgenii Belykh   +9 more
doaj   +1 more source

Characterization of the far-red fluorescent probe MitoView 633 for dynamic mitochondrial membrane potential measurement

open access: yesFrontiers in Physiology, 2023
Introduction: MitoView 633, a far-red fluorescent dye, exhibits the ability to accumulate within mitochondria in a membrane potential-dependent manner, as described by the Nernst equation.
Patrick Ernst   +6 more
doaj   +1 more source

MEMS Actuators for Optical Microendoscopy

open access: yesMicromachines, 2019
Growing demands for affordable, portable, and reliable optical microendoscopic imaging devices are attracting research institutes and industries to find new manufacturing methods.
Zhen Qiu, Wibool Piyawattanametha
doaj   +1 more source

Self-Supervised Joint Learning for pCLE Image Denoising

open access: yesSensors
Probe-based confocal laser endoscopy (pCLE) has emerged as a powerful tool for disease diagnosis, yet it faces challenges such as the formation of hexagonal patterns in images due to the inherent characteristics of fiber bundles.
Kun Yang   +4 more
doaj   +1 more source

Time-Domain Full-Field Confocal Optical Coherence Tomography with Digital Scanning

open access: yesPhotonics
Full-field optical coherence tomography (FF-OCT) is a fast, en face interferometric technique that allows imaging inside a scattering tissue with high spatial resolution.
Danielis Rutkauskas   +2 more
doaj   +1 more source

Residual tail twisting in ascidian larvae is stabilized by asymmetric myofibrils that resist bilateral symmetry restoration

open access: yesFEBS Letters, EarlyView.
Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure   +3 more
wiley   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

open access: yesFEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa   +2 more
wiley   +1 more source

Observation of Real Contact Area Using Laser Microscope with Wide Field of View

open access: yesJournal of Advanced Mechanical Design, Systems, and Manufacturing, 2011
In tribology, a contact condition between mating surfaces is very important to reduce friction and raise their lifetime. Direct observation of apparent contact area is necessary for a better understanding of the contact condition.
Isami NITTA   +3 more
doaj   +1 more source

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