Results 31 to 40 of about 563,943 (268)

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Confocal Laser Endomicroscopy [PDF]

Gastroenterology Research and Practice, 2012
Confocal laser endomicroscopy (CLE) is a newly developed endoscopic technique that enables imaging of the mucosal layer during endoscopy at a subcellular level of resolution. The method can therefore be used for the assessment of changes in vascular architecture, connective tissue, and cellular components in the mucosa, enabling endoscopists to collect
DE PALMA, GIOVANNI DOMENICO, Wallace MB, Giovannini M.   +2 more
openaire   +4 more sources

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Virtual Histology Staining of Skin Tissue using Ex Vivo Confocal Microscopy and Deep Learning [PDF]

Journal of Biomedical Physics and Engineering
Background: The use of Hematoxylin-and-Eosin (H&E) staining is widely accepted as the most reliable method for diagnosing pathological tissues. However, the conventional H&E staining process for tissue sections is time-consuming and requires significant ...
Mahmoud Bagheri, Alireza Ghanadan, Mobin Saboohi, Maryam Daneshpazhooh, Fatemeh Atyabi, Marjaneh Hejazi   +5 more
doaj   +1 more source

Ex Vivo Murine Skin Model for B. burgdorferi Biofilm

Antibiotics, 2020
Borrelia burgdorferi, the causative agent of Lyme disease, has been recently shown to form biofilm structures in vitro and in vivo. Biofilms are tightly clustered microbes characterized as resistant aggregations that allow bacteria to withstand harsh ...
Jason P. Torres, Alireza G. Senejani, Gauri Gaur, Michael Oldakowski, Krithika Murali, Eva Sapi   +5 more
doaj   +1 more source

Spatial Correlations and Distribution of Competence Gene Expression in Biofilms of Streptococcus mutans

Frontiers in Microbiology, 2021
Streptococcus mutans is an important pathogen in the human oral biofilm. It expresses virulent behaviors that are linked to its genetic competence regulon, which is controlled by comX. Expression of comX is modulated by two diffusible signaling peptides,
Ivan P. Ishkov, Justin R. Kaspar, Stephen J. Hagen   +2 more
doaj   +1 more source

The ubiquitin ligase RNF115 is required for the clearance of damaged lysosomes

FEBS Letters, EarlyView.
Upon lysosomal rupture, an E3 ubiquitin ligase RNF115 translocates from the cytosol to the damaged lysosomal membrane. Moreover, RNF115 depletion impairs the clearance of damaged lysosomes, identifying it as a key regulator of lysosomal quality control.
Sae Nakanaga, Toshiki Takahashi, Akiko Kuma, Hiroyuki Kawahara   +3 more
wiley   +1 more source

Endoscopic Confocal Imaging [PDF]

Clinical Gastroenterology and Hepatology, 2005
In vivo fluorescence endomicroscopy is a newly developed diagnostic tool enabling virtual in vivo histology of the mucosal layer during ongoing endoscopy. This review summarizes currently available data about the technique and clinical use of confocal endomicroscopy.
Ralf, Kiesslich, Markus F, Neurath
openaire   +2 more sources

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

Algoritmo de detección de líneas aplicado a la mejora de los procesos de calibración del microscopio LEXT OLYMPUS.

Tecnología y Ciencia, 2021
En este trabajo se presenta un método para procesar imágenes para optimizar la calibración de microscopios ópticos Lext Olympus con plantillas. El algoritmo propuesto ya funciona en las escalas de los autocolimadores Nikon, que son similares a las ...
Guillermo Juan Bergues, Clemar Schürrer, Juan Caselles, Nancy Brambilla   +3 more
doaj   +1 more source