Results 71 to 80 of about 563,943 (268)

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Fluorochrome separation by fluorescence lifetime phasor analysis in confocal and STED microscopy

Methods in Microscopy
In fluorescence microscopy, discrimination of fluorochromes in multi-color labeling was originally based on the emission spectrum only, then on emission and distinct excitation wavelengths.
Gonzalez Pisfil Mariano, Dietzel Steffen
doaj   +1 more source

Ultrastructural Analysis of Johnston’s Organ and Brain Organization in Philaenus spumarius (Hemiptera: Aphrophoridae)

Insects
Insects rely on a variety of sensory cues for orientation, with antennae playing a central role in receiving and transmitting information about the environment.
Milos Sevarika, Christoph Kleineidam, Roberto Romani   +2 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Enhanced chitosan fibres for skin regeneration: solution blow spinning and incorporation with platelet lysate and tannic acid

Materials Research Express
In this study, we developed and characterised enhanced chitosan/polyethylene oxide (PEO) nanofibre scaffolds using solution blow spinning (SBS) for potential application in skin tissue engineering.
Håvard J Haugen, David Coelho, Nguyen D Tien, Tianxiang Geng, Jonny J Blaker   +4 more
doaj   +1 more source

Confocal Neurolasermicroscopy [PDF]

Neurosurgery, 2011
Hans-Georg, Schlosser, Christian, Bojarski   +1 more
openaire   +2 more sources

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Confocal microscopy as an aid in the diagnosis of keratitis due to Candida parapsilosis [PDF]

Revista Brasileira de Oftalmologia
To describe a case of treatment-resistant corneal infiltrate in a patient 4 months after penetrating keratoplasty. The etiological diagnosis was established through microbiology and in vivo corneal confocal microscopy.
Bianca Luiza Valduga Guareschi, Clainijane Ramalho Borges, Paula Kataguiri, Tadeu Cvintal, Victor Cvintal, Bret Alan Moore, Fabiano Montiani Ferreira   +6 more
doaj   +1 more source