Results 71 to 80 of about 739,261 (262)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

New Endoscopic Imaging Technology Based on MEMS Sensors and Actuators

open access: yesMicromachines, 2017
Over the last decade, optical fiber-based forms of microscopy and endoscopy have extended the realm of applicability for many imaging modalities. Optical fiber-based imaging modalities permit the use of remote illumination sources and enable flexible ...
Zhen Qiu, Wibool Piyawattanamatha
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Confocal-wavefront-shaping-code

open access: yes
Code for the paper "Non-invasive and noise-robust light focusing using confocal wavefront shaping"
Dror Aizik (18112474)
core   +1 more source

Segmentation of skin strata in reflectance confocal microscopy depth stacks

open access: yes, 2015
Reflectance confocal microscopy is an emerging tool for imaging human skin, but currently requires expert human assessment. To overcome the need for human experts it is necessary to develop automated tools for automatically assessing reflectance confocal
Ardigo, Marco   +15 more
core   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

open access: yesMolecules, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Gregor P. C. Drummen   +2 more
doaj   +1 more source

In vivo corneal confocal microscopy in keratoconus

open access: yes, 2005
PURPOSE: To evaluate the corneas of keratoconic subjects using in vivo confocal microscopy. METHODS: Slit scanning confocal microscopy was used to evaluate the central cornea of one eye of each of 29 keratoconic subjects (mean age 31 +/- 10 years; range ...
Hollingsworth, Joanna G.   +2 more
core   +1 more source

Eyelid and Conjunctival Tumors In Vivo Confocal Microscopy

open access: yes, 2020
This Atlas gives the complete expert opinion on the diagnostic features of eyelid and conjunctival tumors (benign and malignant): a state-of-the-art guide with numerous images, useful for both dermatologists and ophthalmologists. This invaluable resource,
Cinotti E.   +3 more
core   +1 more source

Antibodies used for confocal microscopy.

open access: yes, 2021
Antibodies used for confocal microscopy.
Bhawna Yadav (172612)   +6 more
core   +1 more source

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