Results 91 to 100 of about 3,449,175 (207)
A dataset of small protein conformational ensembles from all-atom molecular dynamics simulations
Scientific DataSmall proteins, with more pronounced conformational flexibility, are crucial in biological processes and their function is linked to their high flexibility.
Ying Hu +9 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
FEBS Open Bio, EarlyView.Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Conformational Dynamics in an Organic Ionic Plastic Crystal
, 2017 Conformational Dynamics in an Organic Ionic Plastic ...
Fangfang Chen (13063851) +12 more
core
Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA
FEBS Open Bio, EarlyView.PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro +1 more
wiley +1 more source
Global Conformational Dynamics in Ras [PDF]
, 2008 Ras and its homologues are central to regulation of a multitude of cellular processes. Ras in complex with GTP binds and activates its downstream signaling partners.
Kovrigin, Evgenii L., O\u27Connor, Casey
core
Frontiers in BiophysicsOne of the challenges of modern structural biology is to account for protein structural dynamics. Intrinsically disordered proteins (IDPs), which do not fold spontaneously into a defined conformation, existing, instead, as highly heterogeneous ...
Stefania Brocca +5 more
doaj +1 more source
FEBS Open Bio, EarlyView.IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo +6 more
wiley +1 more source
Conformational Dynamics and Residue Coevolution of the DnaK Structures.
, 2017 Conformational dynamics of the ADP-DnaK structure (pdb id 2KHO) (A) and ATP- DnaK structure (pdb id 4B9Q) (B). Residue-based conformational mobility profiles are annotated and colored according to the subdomain nomenclature as in Fig 1.
Gabrielle Stetz (831541) +1 more
core +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
FEBS Open Bio, EarlyView.HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source