Results 251 to 260 of about 163,659 (277)

Escape from antimicrobial CRISPR-Cas9 in E. coli ST131 depends on the genetic context of the target gene

Morros-Bernaus C, Westley J, Wyrsch ER, Djordjevic SP, Zhang L, Leonard AF, Gaze WH, Sünderhauf D, van Houte S.   +8 more
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Use of genetically prepared enzyme conjugates in enzyme immunoassay

Trends in Biochemical Sciences, 1993
Enzyme immunoassay, using enzymes crosslinked to either antibodies or antigens, has proved a valuable immunological tool for many years. Recently, gene fusion techniques have been used to prepare these enzyme conjugates. This method may be especially advantageous in cases where (1) the antigen is difficult and costly to obtain in large quantities or (2)
C, Lindbladh, K, Mosbach, L, Bülow
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Conjugation and Genetic Exchange in Enterococci

2014
The medical importance of the enterococci is closely related to the propensity of these organisms to participate in the horizontal transfer of determinants for antibiotic resistance and virulence. Conjugative plasmids in enterococci tend to fall into two main groups.
Don B. Clewell, Gary M. Dunny
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Genetically Engineered Epidermal Growth Factor Conjugate Crosses Cell Membrane

Applied Biochemistry and Biotechnology, 2012
Epidermal growth factor (EGF) is a well-known pleiotropic growth factor in mammal, and has been attempted to be used in many different fields. However, the application of EGF is limited because of its poor cell permeation. In order to increase membrane permeation ability of EGF, a genetically modified recombinant EGF (GST-TAT-EGF) was prepared through ...
Baoquan, Zhao, Yanru, Guo, Ailing, Fu
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Genetic Evidence of Protein Transfer during Bacterial Conjugation

Plasmid, 1999
DNA can be transferred from eubacteria to at least plants, fungi, and all other eubacteria by related, plasmid-mediated conjugation. Little is known about the biochemistry of intraspecies or interspecies DNA transfer. Even less is known about what other molecules may accompany the DNA, or the direct or inheritable effects on recipients of these escort ...
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[Genetic control of plasmid R6K conjugativity].

Genetika, 1982
The regions determining conjugation ability of plasmid R6K were localized by means of deletion mutants obtained in vitro and in vivo and Tra- mutants induced by integration of transposons Tn5, Tn7 and Tn9 into different DNA sites of the conjugative deletion mutant pAS3.
E G, Abalakina, L Z, Iakubov, B A, Rebentish, A I, Stepanov   +3 more
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On the mechanism of genetic transfer during conjugation ofEscherichia coli

Journal of Cellular Physiology, 1967
It seems likely that the transfer of genetic material observed upon mating of male strains of Escherichia coli K12 is due to (or is at least concomitant with) a particular replication of the donor DNA. The detailed mechanism of genetic transfer is discussed in the light of different, recently published experimental results.
F, Cuzin, G, Buttin, F, Jacob
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Genetic Organization and Expression of Non-Conjugative Plasmids

1981
Non-conjugative plasmids are plasmids that are not able to transfer themselves to other cells without the help of a conjugative system provided by the large, so-called conjugative plasmids. Non-conjugative plasmids are small plasmids. Their M.W. generally does not exceed 10 Megadaltons. Furthermore, they are multicopy plasmids; that means that they are
H. John, J. Nijkamp, Eduard Veltkamp
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