Results 81 to 90 of about 163,659 (277)

Genetic Dissection of a Prevalent Plasmid-Encoded Conjugation System in Lactococcus lactis

Frontiers in Microbiology, 2021
Plasmid pNP40, which was first identified nearly 40 years ago in Lactococcus lactis subsp. lactis biovar diacetylactis DRC3, encodes functions such as heavy metal-, bacteriophage-, and nisin-resistance, as well as plasmid transfer ability by conjugation.
Guillermo Ortiz Charneco, Philip Kelleher, Andrius Buivydas, Hugo Streekstra, Emiel Ver Loren van Themaat, Paul P. de Waal, Jennifer Mahony, Douwe van Sinderen   +7 more
doaj   +1 more source

Development of an improved Pseudoalteromonas haloplanktis TAC125 strain for recombinant protein secretion at low temperature [PDF]

, 2008
Background: In a previous paper, we reported the accomplishment of a cold gene-expression system for the recombinant secretion of heterologous proteins in Pseudoalteromonas haloplanktis TAC125.
CIRULLI C., DE VIZIO D., PARRILLI, ERMENEGILDA, TUTINO, MARIA LUISA   +3 more
core   +1 more source

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

Methods for the genetic manipulation of marine bacteria

Electronic Journal of Biotechnology, 2018
Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications. This procedure can be applied to explore the
Zahraa Zeaiter, Francesca Mapelli, Elena Crotti, Sara Borin   +3 more
doaj   +1 more source

Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10

FEBS Letters, EarlyView.
Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang, Satoshi Hashimoto, Sota Ito, Chisato Kikuguchi, Miho Hoshi, Kiyoshi Yamaguchi, Yoichi Furukawa, Toru Suzuki, Toshifumi Inada   +8 more
wiley   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Influence of Cultivation Conditions on the Transmission of Antibiotic Resistance Genes in Vibrio cholera

Антибиотики и Химиотерапия
The growth of antibiotic resistance necessitates studying the processes of acquisition and loss of genetic elements responsible for resistance. The aim of the study was to investigate the effect of temperature, biofilm formation, and antibiotics on the ...
N. A. Selyanskaya, S. V. Titova, E. A. Menshikova, S. O. Vodopyanov, V. D. Kruglikov   +4 more
doaj   +1 more source

Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar Typhimurium isolated from human and animal sources in Hungary

Veterinární Medicína, 2005
The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar ...
N. Nogrady, I. Gado, P. Zsolt Fekete, J. Paszti   +3 more
doaj   +1 more source

Yeasts [PDF]

, 2018
Yeasts are a group of eukaryotic microfungi with a well-defined cell wall whose growth is either entirely unicellular or a combination of hyphal and unicellular reproduction. The approximately 1500 known yeast species belong to two distinct fungal phyla,
Lachance, Marc-Andre, Walker, Graeme M.
core   +2 more sources

The role of histone modifications in transcription regulation upon DNA damage

FEBS Letters, EarlyView.
This review discusses the critical role of histone modifications in regulating gene expression during the DNA damage response (DDR). By modulating chromatin structure and recruiting repair factors, these post‐translational modifications fine‐tune transcriptional programmes to maintain genomic stability.
Angelina Job Kolady, Siyao Wang
wiley   +1 more source