Results 121 to 130 of about 82,240 (227)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Hungarian Validation of the Individualized Neuromuscular Quality-of-Life Questionnaire (INQoL) in Adult Patients with Muscular Diseases. [PDF]

Neurol Int
Ruszin-Perecz B, Héjas R, Makai A, Hajdu N, Jedlicska D, Ruszin-Perecz B, Sipos A, Pál E, Varga D.   +8 more
europepmc   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Cross-cultural adaptation and validation of a Moroccan Arabic version of the Hooked on Nicotine Checklist. [PDF]

Front Public Health
Moutawakkil SG, El-Ammari A, El Gnaoui S, El Rhazi K, Zarrouq B.   +4 more
europepmc   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Psychometric properties of the Swedish versions of Spinal Cord Independence Measure IV (SCIM IV) and Self-report (SCIM-SR) in inpatient and outpatient rehabilitation settings. [PDF]

Spinal Cord
Antepohl U, Butler Forslund E, Flank P, Holmlund L, Antepohl W, Levi R, STRIVE-SCI Consortium, Divanoglou A, Jörgensen S.   +8 more
europepmc   +1 more source

AscF in the mycobacterial CIII–CIV supercomplex lacks metal and nucleotide binding but links malate oxidation to respiration

FEBS Letters, EarlyView.
The mycobacterial CIII‐CIV respiratory supercomplex is an obligate assembly, encompassing several subunits of unknown functions. We have characterized the intracellular subunit AscF, and show that it is unlikely to be a sensor for metals or nucleotides, but is required for growth on nonfermentable energy sources, and likely works as an adapter for ...
Eni Rile, Riccardo Diamanti, Daniel Lundin, Soni Sharma, Robin Lissner, Coenraad P. Kuil, Martin Högbom, Dirk Bald, Dan Sjöstrand   +8 more
wiley   +1 more source

Praxis function in the ADL Observation Scale: Spanish cross-cultural adaptation and psychometric properties. [PDF]

PLoS One
Sánchez-Bermejo L, Milla-Ortega PJ, Pérez-Mármol JM.   +2 more
europepmc   +1 more source

Identification of the plant mitochondrial OrfX protein: A mass spectrometry approach

FEBS Letters, EarlyView.
The mitochondrial genome of plants contains an open reading frame, orfx, which encodes a rare protein that has so far escaped mass spectrometric detection. The protein resembles the c‐subunit of bacterial twin‐arginine‐motif‐dependent protein translocases (TatC).
Matthias Döring, Jan de Jonge, Hans‐Peter Braun, Nils Rugen   +3 more
wiley   +1 more source

Diversity of self-construal and behavioral motivation system in Indonesia: a comparative study in Torajan and Makassarese communities in South Sulawesi. [PDF]

BMC Psychol
Istiqlal T, Mumang AA, Liaury K, Tanra AJ, Uchida Y, Ishida T, Shimizu-Furusawa H, Yusuf I, Furusawa T.   +8 more
europepmc   +1 more source