Results 61 to 70 of about 75,381 (260)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

The Needle in the Haystack: Uncovering the First Whale Shark (Rhincodon typus) Aggregation in the Coral Sea

Ecology and Evolution
Aggregations are key events, supporting critical ecological and biological functions in many species. For highly mobile and elusive species, aggregations often provide the only feasible opportunities for research.
Ingo B. Miller, Richard Fitzpatrick, Kátya G. Abrantes, Bradley Norman, Simon J. Pierce, Mark V. Erdmann, Lisa A. Hoopes, Christine Dudgeon, Matthew D. Dunbabin, Alistair D. M. Dove, Robin J. Beaman, Samantha D. Reynolds, Christopher Rohner, Samuel M. Williams, David Paton, Sonny Lewis, Adam Barnett   +16 more
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

FEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young, Omar Arias‐Gaguancela, Fiona Gibson, Morgan Grainger, Jodie Score, Amit Nathubhai, Mauricio Cafiero, Lee Richard Machado   +7 more
wiley   +1 more source

S192: SINGLE-CELL RNA PROFILING OF MYELOFIBROSIS PATIENTS REVEALS PELABRESIB-INDUCED DECREASE OF MEGAKARYOCYTIC PROGENITORS AND NORMALIZATION OF CD4+ T CELLS IN PERIPHERAL BLOOD

HemaSphere, 2022
O. Zavidij, N. J. Haradhvala, R. Meyer, J. Cui, S. Verstovsek, S. Oh, A. Mead, P. Taverna   +7 more
doaj   +1 more source

Technical Design of a Low-Latitude Satellite Constellation for Ocean Observation with a Focus on Hainan Province, China

Sensors
Acquiring high-quality images from space at low-latitude areas is challenging due to the orbital requirements of the satellites and the frequent cloud coverage.
Lei Wang, Tianliang Yang, Tianyue Wang, Chengyi Wang, Ningyang Li, Xiao-Ming Li   +5 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Noncoherent unitary space-time design base on Grassmann manifold

Tongxin xuebao, 2013
A novel search technique was presented for designing noncoherent unitary space-time constellation.First of all,a unitary matrix framework based on Grassmann manifold was defined.Under the constraint of this framework,a dis-tance threshold was set by ...
Da-wei FU, Li PENG, Li-jiao WANG, Qiu-ping PENG   +3 more
doaj