Results 61 to 70 of about 419,968 (313)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Mapping the e-business profile and trends in cost management in the UK construction industry

, 2012
The advancement of e-business applications and IT infrastructure has had massive impact on construction business processes over the last decade. The added effects of globalisation coupled with global economic recession have forced businesses to implement
Zhou, Lei, Perera, Srinath (R18622), Perera, Srinath, Park, Rachael, Rodrigo, Anushi, Udeaja, Chika   +5 more
core  

Development of cost benefit analysis model of accident prevention on construction projects [PDF]

, 2009
A thesis submitted in partial fulfilment of the requirements of the University of Wolverhampton for the degree of Doctor of PhilosophyThe Health and Safety Executive estimated the annual cost to British employers and other duty holders failing to comply ...
Ikpe, Elias Okede
core  

Identifying gene expression signatures for risk stratification of postoperative adjuvant chemotherapy in colorectal cancer

FEBS Open Bio, EarlyView.
A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo, Keisuke Okuno, Shuichi Watanabe, Sakiko Oba, Hiroyasu Kagawa, Masanori Tokunaga, Daisuke Ban, Yusuke Kinugasa   +7 more
wiley   +1 more source

Implementing design requirements: the mitigating factor on construction cost understimation

, 2018
The construction project cost estimate has been viewed as a mere budgetary allocation, in which accuracy remains uncertain. Cost underestimation have negative construction to construction projects and have not been adequately explored with the objective
Ndihokubwayo, Ruben, Fapohunda, Julius Ayodeji, Awosina, Abiodun   +2 more
core   +1 more source

Report : enabling team collaboration with pervasive and mobile computing : cost benefit analysis methodology

, 2006
Construction is an information intensive industry in which the accuracy and\ud timeliness of information is paramount. It observed that the main\ud communication issue in construction is to provide a method to exchange\ud data between the site operation,

core  

Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs

FEBS Open Bio, EarlyView.
Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner, Hainan Zhu, Carol Christine Bosholm, Heuy‐Ching Wang, Tracy Criswell, Anthony Atala, Jian‐Xing Ma, Yuanyuan Zhang   +7 more
wiley   +1 more source

The Influence of Vertical Ground Motion on the Design of Common R/C Frames

Applied Sciences
In this article, the response of reinforced concrete frames concurrently subjected to both horizontal and vertical seismic motions is assessed. The article is not limited to the variation in response quantities but aims to identify which specific design ...
Grigorios Manoukas, Vasilios Tsiggelis
doaj   +1 more source

First interim report : cost of tendering

, 2008
This is the first interim report on the Cost of Tendering component of the Best Value project. This report provides some insight from ‘cost of tendering’ literature and\ud discussions with CRC partners.\ud With the completion of this scoping project ...

core  

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source