Results 61 to 70 of about 63,450 (264)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Influence of premixed swirl-stabilized flame on the cooling performance of an effusion-cooled combustor liner

Case Studies in Thermal Engineering
The impact of premixed swirl-stabilized flame on the cooling performance of an effusion-cooled combustor liner perforated by cylindrical and fan-shaped holes is investigated under varying blowing ratios.
Xiang Lu, Yongbin Ji, Bing Ge, Shusheng Zang   +3 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Numerical Investigation on Film Cooling Mechanism with Different Coolant Delivery Configurations

Journal of Applied Fluid Mechanics, 2021
Kidney vortex has significant impact on film cooling effectiveness, and different kinds of film cooling hole geometry and configuration are developed to weaken or eliminate kidney vortex.
Y. T. Jiang, H. F. Deng, X. L. You, H. J. Zhao, G. Q. Yue   +4 more
doaj  

SPG4 and Dementia: Expanding the Clinical Spectrum

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza, Arun Meyyazhagan, Eliseo Picchi, Gustavo Ribas, Ingrid Faber, Ryosuke Miyamoto, Preethi Basavaraju, Paolo Eusebi, Haripriya Kuchi Bhotla, Mario Stasi, Fabrizio Gaudiello, Francesco Patti, Filippo Maria Santorelli, Marcondes Cavalcante França Jr, José Luiz Pedroso, Orlando Graziani Povoas Barsottini, Hélio Afonso Ghizoni Teive, Peter Henry St George‐Hyslop, Toshitaka Kawarai, Antonio Orlacchio   +19 more
wiley   +1 more source

Design and Experimental Study of Turbine Blade Cooling Structure for an Engine

Aerospace
To address issues such as leading-edge and trailing-edge ablation and cracking of turbine blades during operation in an engine, this study integrates the characteristics of additive manufacturing technology and utilizes a comprehensive simulation and ...
Jintao Jiang, Suyan Dong, Rongxiao Dong, Zhisheng Wang, Yi Fu, Penghui Liu, Wei Fan   +6 more
doaj   +1 more source

The Role of Calcitonin Gene‐Related Peptide in High‐Altitude Headache: A Prospective Field Study

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective High‐altitude headache (HAH) is a common neurological condition associated with rapid ascent to high altitude. The pathophysiological mechanisms underlying HAH remain incompletely understood. Calcitonin gene‐related peptide (CGRP), a neuropeptide implicated in migraine pathophysiology, may play a key role in the pathophysiology of ...
Roman Schniepp, Ludwig Karrasch, Helena Breitenstein, Andreas Straube, Katharina Kamm   +4 more
wiley   +1 more source

Quantitative Assessment of Cooling Methods for Electrical Machines in Aircraft Drives

IEEE Access
Transformation of the aviation sector towards climate-neutral solutions such as electrification is urgent. Aviation electrification requires reasoned selection of suitable technologies which has to meet strict requirements and standards.
Karunakar Reddy Konda, Jonas Franzki, Dikshant Sharma, Paul Konig, Akilan Mathiazhagan, Markus Henke, Klaus Hoschler   +6 more
doaj   +1 more source

Combined Thermal Effect of Cool Roof and Cool Façade on a Prototype Building

Energy Procedia, 2015
AbstractRecently, huge efforts were made to develop new passive solutions for optimizing building summer thermal-energy behavior. While cool roofs are well investigated, a lack of knowledge is detected about the benefits deriving from the combination of cool roofs and cool façades.
PISELLO, ANNA LAURA, CASTALDO, VERONICA LUCIA, PISELLI, CRISTINA, PIGNATTA, GLORIA, COTANA, Franco   +4 more
openaire   +4 more sources

Added Prognostic Value of EEG Reactivity in Comatose Patients Following Cardiac Arrest

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives To evaluate the added prognostic value of EEG reactivity for favorable outcome compared with background analysis during and after targeted temperature management (TTM). Methods Prospective observational cohort study of comatose post–cardiac arrest patients admitted to a single academic center between 2017 and 2022, all undergoing ...
Sarah Caroyer, Marie Obrecht, Charlotte de Maeseneire, Charlotte Damien, Chantal Depondt, Lorenzo Ferlini, Estelle Rikir, Filippo Annoni, Fabio Silvio Taccone, Nicolas Mavroudakis, Benjamin Legros, Nicolas Gaspard   +11 more
wiley   +1 more source