Results 21 to 30 of about 4,940,025 (211)

Telomerase immortalization of human corneal endothelial cells yields functional hexagonal monolayers. [PDF]

PLoS ONE, 2012
Human corneal endothelial cells (HCEnCs) form a monolayer of hexagonal cells whose main function is to maintain corneal clarity by regulating corneal hydration. HCEnCs are derived from neural crest and are arrested in the post-mitotic state.
Thore Schmedt, Yuming Chen, Tracy T Nguyen, Shimin Li, Joseph A Bonanno, Ula V Jurkunas   +5 more
doaj   +1 more source

Vitrification of Corneal Endothelial Cells in a Monolayer

Journal of Bioscience and Bioengineering, 2008
An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.
Fan, W, Ma, X, Liu, T, Cui, Z
openaire   +2 more sources

Treatment of corneal endothelial damage in a rabbit model with a bioengineered graft using human decellularized corneal lamina and cultured human corneal endothelium.

PLoS ONE, 2019
ObjectiveWe aimed to investigate the functionality of human decellularized stromal laminas seeded with cultured human corneal endothelial cells as a tissue engineered endothelial graft (TEEK) construct to perform endothelial keratoplasty in an animal ...
Francisco Arnalich-Montiel, Adrian Moratilla, Sherezade Fuentes-Julián, Veronica Aparicio, Marta Cadenas Martin, Gary Peh, Jodhbir S Mehta, Khadijah Adnan, Laura Porrua, Ane Pérez-Sarriegui, Maria P De Miguel   +10 more
doaj   +1 more source

Biomaterials for corneal endothelial cell culture and tissue engineering

Journal of Tissue Engineering, 2021
The corneal endothelium is the posterior monolayer of cells that are responsible for maintaining overall transparency of the avascular corneal tissue via pump function.
Mohit Parekh, Vito Romano, Kareem Hassanin, Valeria Testa, Rintra Wongvisavavit, Stefano Ferrari, Atikah Haneef, Colin Willoughby, Diego Ponzin, Vishal Jhanji, Namrata Sharma, Julie Daniels, Stephen B Kaye, Sajjad Ahmad, Hannah J Levis   +14 more
doaj   +1 more source

Review: corneal endothelial cell derivation methods from ES/iPS cells

Inflammation and Regeneration, 2019
Globally, approximately 12.7 million people are awaiting a transplantation, while only 185,000 cases of corneal transplantation are performed in a year. Corneal endothelial dysfunction (bullous keratopathy) due to Fuchs’ corneal endothelial dystrophy, or
Shin Hatou, Shigeto Shimmura
doaj   +1 more source

Genetic mutations and molecular mechanisms of Fuchs endothelial corneal dystrophy

Eye and Vision, 2021
Background Fuchs endothelial corneal dystrophy is a hereditary disease and the most frequent cause of corneal transplantation in the worldwide. Its main clinical signs are an accelerated decrease in the number of endothelial cells, thickening of Descemet’
Xuerui Liu, Tao Zheng, Chuchu Zhao, Yi Zhang, Hanruo Liu, Liyuan Wang, Ping Liu   +6 more
doaj   +1 more source

Acanthamoeba induces cell-cycle arrest in host cells [PDF]

, 2004
Acanthamoeba can cause fatal granulomatous amoebic encephalitis (GAE) and eye keratitis. However, the pathogenesis and pathophysiology of these emerging diseases remain unclear.
Alsam, S., Stins, M., Khan, Naveed Ahmed, Sissons, J., Kim, K.S., Jayasekera, S.   +5 more
core   +1 more source

The mucinous layer of corneal endothelial cells

Yonsei Medical Journal, 2000
The goal of this study was to characterize the morphology of the mucinous layer on rabbit, bovine, owl, and human corneal endothelial cells.Corneoscleral buttons were fixed using cetylpyridinium chloride to stabilize "mucus" and the tissue was prepared for transmission electron microscopy.
E K, Kim, S M, Cristol, H L, Kim, S J, Kang, J W, Park, H F, Edelhauser   +5 more
openaire   +2 more sources

Transcriptomic Analysis of Cultured Corneal Endothelial Cells as a Validation for Their Use in Cell Replacement Therapy

Cell Transplantation, 2016
The corneal endothelium plays a primary role in maintaining corneal homeostasis and clarity and must be surgically replaced with allogenic donor corneal endothelium in the event of visually significant dysfunction.
Ricardo F. Frausto, Derek J. Le, Anthony J. Aldave M.D.   +2 more
doaj   +1 more source

Advances in culture, expansion and mechanistic studies of corneal endothelial cells: a systematic review

Journal of Biomedical Science, 2019
Human corneal endothelial cells are notorious for their restricted proliferative ability in vivo and in vitro. Hence, injury or dysfunction of these cells may easily result in blindness.
Shuangling Chen, Qin Zhu, Hong Sun, Yuan Zhang, Sean Tighe, Li Xu, Yingting Zhu   +6 more
doaj   +1 more source